Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Self-assembly of a triple-zwitterion in polar solutions: hierarchical formation of nanostructures.

Soft matter·2026

Same author

Structure-Reactivity Relationships in <i>N</i>-Methylpyridinium Aldoxime Isomers: Comparative Experimental and Computational Studies.

International journal of molecular sciences·2026

Same author

Combined mass spectrometry and computational analysis of cyanine dyes and their structural analogues.

RSC advances·2026

Same author

Preparation and Application of Sulfamethoxazole-Imprinted Polymer on Solid-Phase Extraction of Pharmaceuticals from Water.

Polymers·2025

Same author

Molecular Pathways Affected by Sulfonylpurine Derivatives in 2D and 3D HeLa Cell Models.

Molecules (Basel, Switzerland)·2025

Same author

Induction of helical chirality in tetrahedral transition metal complexes with non-bridging monodentate oxazolines.

Dalton transactions (Cambridge, England : 2003)·2025

Search research articles

Related Experiment Video

Updated: Jun 26, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Distinctive Nucleic Acid Recognition by Lysine-Embedded Phenanthridine Peptides.

Josipa Matić¹, Patryciusz Piotrowski², Lucija Vrban³

¹Laboratory for Biomolecular Interactions and Spectroscopy, Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička Cesta 54, 10000 Zagreb, Croatia.

International Journal of Molecular Sciences

|May 11, 2024

Summary

This summary is machine-generated.

New phenanthridine peptide derivatives act as sensitive probes for DNA and RNA. Lysine-containing compounds show enhanced binding and selective fluorescence, enabling polynucleotide recognition.

Keywords:

DNA/RNA recognition molecular dynamics simulations phenanthridine peptides spectrophotometric AT- and GC-base pair probe

More Related Videos

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Related Experiment Videos

Last Updated: Jun 26, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Area of Science:

Chemical Biology
Molecular Biology
Spectroscopy

Background:

Phenanthridine derivatives are explored for their nucleic acid binding properties.
Understanding molecular interactions is key for developing diagnostic probes.

Purpose of the Study:

Synthesize and characterize novel phenanthridine peptide derivatives.
Investigate their potential as spectrophotometric probes for DNA and RNA.
Evaluate the impact of structural modifications on binding affinity and selectivity.

Main Methods:

Synthesis of phenanthridine peptide derivatives (compounds 19, 22, 23).
Spectroscopic analysis (UV/Vis, circular dichroism).
Mass spectrometry and computational modeling.
Binding studies with various polynucleotides.

Main Results:

Compounds 22 and 23, containing lysine, exhibited stronger DNA/RNA binding than compound 19.
Compound 23 showed selective fluorimetric response and induced triplex formation with AT/ATT polynucleotides.
A high binding constant (K = 2.5 × 10^7 M^-1) was achieved with AT and ATT polynucleotides.
Selective recognition of ds-DNA, ds-RNA, and GC-rich DNA was observed via induced CD signals.

Conclusions:

Lysine incorporation enhances binding affinity of phenanthridine derivatives to nucleic acids.
Compound 23 serves as a selective probe for specific DNA/RNA sequences and structures.
These derivatives hold promise for applications in molecular diagnostics and biophysical studies.