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MicroRNA-135b-5p Is a Pathologic Biomarker in the Endothelial Cells of Arteriovenous Malformations
Joon Seok Lee1, Gyeonghwa Kim2,3, Jong Ho Lee1
1Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Abstract:
Arteriovenous malformations (AVMs) are congenital vascular anomalies with a poor prognosis. AVMs are considered intractable diseases, as there is no established approach for early diagnosis and treatment. Therefore, this study aimed to provide new evidence by analyzing microRNAs (miRNAs) associated with AVM. We present fundamental evidence for the early diagnosis and treatment of AVM by analyzing miRNAs in the endothelial cells of AVMs. This study performed sequencing and validation of miRNAs in endothelial cells from normal and AVM tissues. Five upregulated and two downregulated miRNAs were subsequently analyzed under hypoxia and vascular endothelial growth factor (VEGF) treatment by one-way analysis of variance (ANOVA). Under hypoxic conditions, miR-135b-5p was significantly upregulated in the AVM compared to that under normal conditions, corresponding to increased endothelial activity (p-value = 0.0238). VEGF treatment showed no significant increase in miR-135b-5p under normal conditions, however, a surge in AVM was observed. Under both hypoxia and VEGF treatment, comparison indicated a downregulation of miR-135b-5p in AVM. Therefore, miR-135b-5p was assumed to affect the pathophysiological process of AVM and might play a vital role as a potential biomarker of AVMs for application related to diagnosis and treatment.
Insights
Researchers identified microRNAs (miRNAs) as potential biomarkers for arteriovenous malformations (AVMs). MiR-135b-5p shows promise for early AVM diagnosis and treatment strategies.
Area of Science:
- Vascular Biology
- Molecular Diagnostics
- Genomics
Background:
- Arteriovenous malformations (AVMs) are congenital vascular anomalies with poor prognoses and lack established early diagnostic and treatment approaches.
- Current understanding of AVM pathophysiology is limited, necessitating novel molecular targets for intervention.
Purpose of the Study:
- To investigate the role of microRNAs (miRNAs) in the development and progression of AVMs.
- To identify potential miRNA biomarkers for early diagnosis and therapeutic strategies for AVMs.
Main Methods:
- Sequencing and validation of miRNAs in endothelial cells from normal and AVM tissues.
- Analysis of miRNA expression under hypoxic conditions and vascular endothelial growth factor (VEGF) treatment using one-way ANOVA.
- Statistical analysis of differential miRNA expression (p-value = 0.0238 for miR-135b-5p).
Main Results:
- Five miRNAs were found to be upregulated, and two were downregulated in AVM endothelial cells.
- miR-135b-5p was significantly upregulated under hypoxic conditions in AVMs, correlating with increased endothelial activity.
- VEGF treatment induced a surge in miR-135b-5p in AVMs, while hypoxia and VEGF combined led to its downregulation in AVMs.
Conclusions:
- miR-135b-5p is implicated in the pathophysiological processes of AVMs.
- miR-135b-5p holds potential as a biomarker for the diagnosis and treatment of AVMs.
- Further research is warranted to elucidate the precise mechanisms and therapeutic applications of miR-135b-5p in AVMs.
