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Related Experiment Video

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Roseburia intestinalis Supplementation Could Reverse the Learning and Memory Impairment and m6A Methylation

Xuejing Sun1, Cui Zhou1, Mengwei Ju1

  • 1Beijing Key Laboratory of Environmental Toxicology, School of Public Health, Capital Medical University, Beijing 100069, China.

Nutrients
|May 11, 2024
PubMed
Summary
This summary is machine-generated.

27-Hydroxycholesterol (27-OHC) disrupts gut microbiota and brain m6A methylation, impairing memory. Supplementing with Roseburia intestinalis reverses these effects, offering neuroprotection against 27-OHC-induced damage.

Keywords:
27-hydroxycholesterolRoseburia Intestinalislearning and memory abilitym6A

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Area of Science:

  • Neuroscience
  • Microbiology
  • Molecular Biology

Background:

  • N6-methyladenosine (m6A) methylation abnormalities are implicated in Alzheimer's disease (AD).
  • 27-Hydroxycholesterol (27-OHC) impacts brain m6A methylation and cognitive function.
  • Gut microbiota dysbiosis is a key mediator in 27-OHC-induced cognitive impairment, with reduced *Roseburia intestinalis* abundance observed.

Purpose of the Study:

  • To investigate the association between 27-OHC, gut *Roseburia intestinalis*, and brain m6A modification in learning and memory deficits.
  • To explore the potential of *Roseburia intestinalis* as a therapeutic intervention against 27-OHC-induced neurotoxicity.

Main Methods:

  • Antibiotic cocktail treatment to deplete gut microbiota in C57BL/6J mice.
  • Subcutaneous injection of 27-OHC or saline, followed by gavage of *Roseburia intestinalis* or saline.
  • Measurement of brain 27-OHC levels, gut barrier function, brain m6A modification, and learning/memory performance.

Main Results:

  • 27-OHC significantly impaired gut barrier function, altered m6A methylation enzyme expression, reduced brain cortex m6A levels, and caused learning/memory deficits.
  • *Roseburia intestinalis* supplementation effectively reversed the detrimental effects of 27-OHC on gut barrier, m6A modification, and cognitive function.
  • Reduced abundance of *Roseburia intestinalis* was confirmed in the 27-OHC intervention group.

Conclusions:

  • 27-OHC-induced learning and memory impairment is closely linked to disturbances in brain m6A methylation.
  • *Roseburia intestinalis* demonstrates significant potential as a probiotic to counteract 27-OHC-induced neuroinflammation and cognitive decline.
  • This study provides mechanistic insights into 27-OHC neurotoxicity and highlights *Roseburia intestinalis* for future neuroprotective strategies.