Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Problems in ANA test interpretation: a comparison of two substrates.

J H Rippey, S Carter, P Hood

    Diagnostic Immunology
    |January 1, 1985
    PubMed
    Summary

    Choosing the right substrate for antinuclear antibody (ANA) testing is crucial. HEp-2 slides offer better specificity for systemic lupus erythematosus, while mouse kidney slides detect more nonspecific ANA.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What Does Equitable Co-Production Entail? Three Perspectives.

    Community science·2026
    Same author

    Infantile Diseases Depending upon Inflammation.

    Western journal of medicine and surgery·2024
    Same author

    Glucagon-like peptide-1 secretion in people with versus without type 2 diabetes: a systematic review and meta-analysis of cross-sectional studies.

    Metabolism: clinical and experimental·2022
    Same author

    Testing a Generalizable Machine Learning Workflow for Aquatic Invasive Species on Rainbow Trout (<i>Oncorhynchus mykiss</i>) in Northwest Montana.

    Frontiers in big data·2021
    Same author

    Differential relationships between parent-child DXA and pQCT bone measures: Results from the Southampton Women's Survey.

    Bone·2021
    Same author

    Patient experiences with hidradenitis suppurativa: the Hidradenitis Patient Experience survey.

    Clinical and experimental dermatology·2021

    Area of Science:

    • Immunology
    • Clinical Chemistry
    • Autoimmunity

    Background:

    • Antinuclear antibody (ANA) testing is a key diagnostic tool for autoimmune diseases.
    • Different substrate slides, such as HEp-2 cells and mouse kidney sections, are used for ANA detection.
    • Variations in substrate can influence test results and their interpretation.

    Purpose of the Study:

    • To compare the performance of HEp-2 cell and mouse kidney substrates in ANA testing.
    • To evaluate the impact of substrate choice on end-point titers, pattern production, and clinical correlation.
    • To provide guidance on optimal substrate selection for ANA assays.

    Main Methods:

    • Serum samples were tested for ANA using both HEp-2 cell and mouse kidney substrates.
    • End-point titers and immunofluorescence patterns were recorded.
    • Qualitative results and correlation with clinical status were analyzed.
    • The influence of manufacturer-suggested versus laboratory-established screening titers was assessed.

    Main Results:

    • Significant differences were observed in end-point titers and pattern production between HEp-2 and mouse kidney substrates.
    • Qualitative results and clinical correlation varied significantly when using manufacturer-suggested screening titers.
    • Mouse kidney slides showed higher sensitivity for nonspecific ANA, while HEp-2 slides demonstrated greater specificity for systemic lupus erythematosus (SLE)-associated ANA.
    • Laboratory-established screening titers reduced some observed differences.

    Conclusions:

    • Substrate selection for ANA testing critically impacts sensitivity and specificity.
    • HEp-2 substrates are more specific for SLE, whereas mouse kidney substrates are more sensitive for detecting a broader range of ANA.
    • Standardization of screening titers within laboratories and consideration of substrate are essential for valid interlaboratory comparisons and reliable follow-up testing.

    Related Experiment Videos