Particulate matter-induced gene expression patterns in human-derived cells based on 11 public gene expression...

Area of Science:

• Environmental exposure
• Molecular biology
• Genomics

Background:

• Particulate matter (PM) and house dust mite (HDM) exposure can alter gene expression.
• Genes related to inflammation, oxidative stress, and cell death are affected.
• Investigating PM exposure's impact on gene expression is critical for understanding health effects.

Purpose of the Study:

• To examine gene expression pattern changes induced by PM exposure.
• To identify specific gene sets affected by PM exposure.
• To correlate gene expression changes with respiratory disease risks.

Main Methods:

• Analyzed differentially expressed genes (DEGs) from RNA-seq and microarray datasets.
• Utilized five cell line-based datasets and six human-derived datasets.
• Performed enrichment analysis on identified gene sets.

Main Results:

• Identified two distinct gene sets altered by PM exposure.
• Enrichment analysis revealed terms associated with respiratory diseases.
• Validated PM-induced gene expression patterns in human-derived cells.

Conclusions:

• Two gene sets were identified in PM-exposed cells and confirmed in human cells.
• PM2.5 and HDM exposure may alter genes linked to allergies and other diseases.
• Reducing PM2.5 and HDM exposure is important for disease prevention.