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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
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Generate an AZFa deleted human embryonic stem cell line.

Yuting Zhen1, Qina He2, Chao Sun2

  • 1Key Laboratory of Tropical Translational Medicine of Ministry of Education & Key Laboratory of Brain Science Research Transformation in Tropical Environment of Hainan Province, School of Basic Medicine and Life Sciences, Hainan Medical University, China; Hainan Provincial Key Laboratory for human reproductive medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, 571101, China; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 571101, China; National Center for International Research "China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease" by the Ministry of Science and Technology of China, Haikou, Hainan 571101, China; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 571101, China.

Stem Cell Research
|May 11, 2024
PubMed
Summary

Researchers created a human embryonic stem cell line lacking the Y chromosome Azoospermia factor a (AZFa) region using CRISPR/Cas9. This new cell line is crucial for studying male infertility caused by AZFa deletions.

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Area of Science:

  • Genetics
  • Reproductive Biology
  • Stem Cell Biology

Background:

  • Y chromosome deletions, particularly Azoospermia factor a (AZFa), are linked to congenital non-obstructive azoospermia and male infertility.
  • Modeling AZFa deletions is challenging due to the large size of the deleted region on the Y chromosome.

Purpose of the Study:

  • To establish a human embryonic stem cell line with a precisely deleted AZFa region.
  • To assess the potential of the AZFa-deleted stem cell line for studying male infertility mechanisms.

Main Methods:

  • CRISPR/Cas9 gene editing technology was employed to delete the AZFa region in human embryonic stem cells.
  • The resulting AZFa-deleted stem cell line was analyzed for its differentiation potential.

Main Results:

  • A viable human embryonic stem cell line with a complete AZFa deletion was successfully generated.
  • The AZFa-deleted stem cells demonstrated the capacity to differentiate into ectoderm, mesoderm, and endoderm lineages.

Conclusions:

  • The developed AZFa-deleted human embryonic stem cell line provides a valuable model for investigating the role of AZFa in spermatogenesis and male infertility.
  • This model holds significant potential for advancing research into Y chromosome-related infertility and developing therapeutic strategies.