Related Experiment Video
Updated: Jun 26, 2025
12:52
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
15.8K
HIF-1α facilitates glioma proliferation and invasion by activating pyroptosis signaling axis
Xin-Wei Wang1, Hao Fu2, Ya-Min Zhang3
1The First Central Clinical School, Tianjin Medical University, Tianjin, 300142, China.
Chinese Neurosurgical Journal
|May 11, 2024
Summary
Hypoxia-inducible factor 1-alpha (HIF-1α) promotes glioma cell growth and invasion. This occurs by regulating the pyrolysis pathway, offering a potential new therapeutic target for glioma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Hypoxia-inducible factor 1-alpha (HIF-1α) is implicated in cancer development.
- The specific role of HIF-1α in glioma pathogenesis is not well understood.
Purpose of the Study:
- To investigate the mechanism by which HIF-1α influences glioma progression.
- To explore the potential of targeting HIF-1α for glioma treatment.
Main Methods:
- Quantification of HIF-1α mRNA and protein levels in glioma cells.
- Assessment of glioma cell proliferation and invasion using colony formation, transwell, and wound-healing assays.
- Analysis of inflammatory and pyrolysis-related pathway markers.
Main Results:
- HIF-1α expression is elevated in glioma cells.
- Overexpression of HIF-1α enhances glioma cell proliferation and invasion.
- HIF-1α upregulates inflammatory factors (TNF-α, IL-10, IL-1β) and pyrolysis pathway proteins (NLRP3, ASC, caspase-1, GSDMD, GSDME).
Conclusions:
- HIF-1α promotes glioma cell proliferation and invasion via regulation of the pyrolysis pathway.
- Targeting the HIF-1α-pyrolysis axis presents a potential therapeutic strategy for gliomas.
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