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Identification and Verification of Endoplasmic Reticulum Stress-Related Genes as Novel Signatures for Osteoarthritis

Jia Lv1, Nannan Kou1, Yunxuan Li1

  • 1Department of Trauma Surgery, The Second Affiliated Hospital of Kunming Medical University, 374 Yunnan-Myanmar Avenue, Kunming, 650101, China.

Biochemical Genetics
|May 11, 2024
PubMed
Summary

This study identifies six endoplasmic reticulum stress-related genes (ERS) that are crucial for osteoarthritis (OA) progression. These ERS genes show potential as diagnostic biomarkers for OA and influence immune cell activity.

Keywords:
Bioinformatics analysisEndoplasmic reticulum stressMicroarrayOsteoarthritis

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Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Endoplasmic reticulum stress (ERS) is implicated in osteoarthritis (OA) development.
  • Mechanisms linking ERS to OA pathogenesis require further elucidation.

Purpose of the Study:

  • To investigate the role of ERS-related genes in OA progression.
  • To identify potential biomarkers for OA diagnosis.

Main Methods:

  • Analysis of gene expression profiles from OA patients and normal individuals using GEO datasets.
  • Identification of differentially expressed genes (DEGs) and hub ERS-related genes via bioinformatics analyses (PPI networks, STRING, Venn diagrams).
  • Validation of gene expression and diagnostic potential using RT-qPCR, Western blot, and ROC curve analysis.

Main Results:

  • Six key ERS-associated genes (ATF3, DDX3X, JUN, EIF4A1, KDELR3, VEGFA) were identified and linked to OA progression.
  • Expression patterns of these genes were validated in OA rat tissues.
  • These genes demonstrated high diagnostic value for OA and potential roles in immune responses.

Conclusions:

  • Six ERS-associated genes (ATF3, DDX3X, JUN, EIF4A1, KDELR3, VEGFA) are identified as critical players in OA pathogenesis.
  • These genes represent potential diagnostic biomarkers for osteoarthritis.