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NF-κB-dependent Signaling Pathway

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Updated: May 5, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Characterization and expression profiling of buffalo IFN-lambda family.

Devika Gautam¹, Anil Sindhu², Ashutosh Vats³

¹Animal Genomics Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute (NDRI), Karnal, HR 132001, India; Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonepat, HR 139031, India.

Veterinary Immunology and Immunopathology

|May 12, 2024

Summary

This study details buffalo interferon lambda (IFN-λ) sequences and expression. Interferon lambda 3 (IFN-λ3) shows specific tissue and cell-type responses to viral and bacterial stimuli, highlighting its role in innate immunity.

Keywords:

Buffalo Epithelial Cell Fibroblast Cells IFN-λ3 Innate immunity Interferons

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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

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Published on: March 24, 2015

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Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

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Published on: May 19, 2023

Area of Science:

Immunology
Molecular Biology
Genomics

Background:

Interferon lambda (IFN-λ), a type III interferon, is crucial for innate immunity against viral infections.
IFN-λ signals through JAK-STAT pathways, similar to type I interferons.
Understanding IFN-λ in livestock is vital for disease resistance.

Purpose of the Study:

To determine buffalo IFN-λ sequences and analyze their expression patterns.
To investigate IFN-λ responses to viral (poly I:C) and bacterial (MRSA) stimuli.
To elucidate the role of IFN-λ in buffalo innate immune responses.

Main Methods:

Polymerase chain reaction (PCR) to deduce IFN-λ sequences.
RT-qPCR to analyze gene expression in various tissues and cell types.
Induction with poly I:C and live Staphylococcus aureus (S. aureus).

Main Results:

Full-length sequences for buffalo IFN-λ3, IFN-λ receptors, and a variant of IFN-λ4 were determined; IFN-λ1 is a pseudogene.
IFN-λ3 expression was highest in lungs and monocytes, with cell-type-specific responses to poly I:C.
Endometrial epithelial cells expressed IFN-λ3 following S. aureus infection, indicating a role in bacterial defense.

Conclusions:

Buffalo possess diverse IFN-λ sequences with distinct expression profiles.
IFN-λ3 plays a significant role in both viral and bacterial innate immune responses in buffalo.
Tissue and cell-specific expression patterns highlight the complex regulation of IFN-λ in immune modulation.