Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.0K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Pir-hsa-23533 Promotes Malignancy In Head And Neck Squamous Cell Carcinoma Via Usp7.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Pir-hsa-23533 Promotes Malignancy In Head And Neck Squamous Cell Carcinoma Via Usp7.

Related Experiment Video

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
08:59

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Published on: January 5, 2017

10.6K

PiR-hsa-23533 promotes malignancy in head and neck squamous cell carcinoma via USP7.

Hanlin Hu1, Jingyu Lu2, Mingjin Xu1

  • 1Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, China.

Translational Oncology
|May 12, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

PIWI-interacting RNA (piRNA) hsa-23533 is upregulated in head and neck squamous cell carcinoma (HNSCC), promoting tumor growth by enhancing proliferation and inhibiting apoptosis. This piRNA targets USP7, offering a potential therapeutic avenue for HNSCC.

Keywords:
ApoptosisHead and neck squamous cell carcinomaPiRNAProliferation

More Related Videos

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.5K
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.4K

Related Experiment Videos

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
08:59

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Published on: January 5, 2017

10.6K
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.5K
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.4K

Area of Science:

  • Molecular Oncology
  • Epigenetics
  • RNA Biology

Background:

  • Head and neck squamous cell carcinoma (HNSCC) progression involves genetic and epigenetic alterations.
  • PIWI-interacting RNAs (piRNAs) play a role in tumorigenesis, but their specific functions in HNSCC are not fully understood.

Purpose of the Study:

  • To investigate the role and molecular mechanisms of piRNAs in HNSCC.
  • To identify specific piRNAs involved in HNSCC development and progression.

Main Methods:

  • RNA sequencing to identify differentially expressed piRNAs.
  • Quantitative real-time PCR and RNA in situ hybridization to assess piR-hsa-23533 expression.
  • In vitro and in vivo assays to evaluate the functional impact of piR-hsa-23533 on HNSCC cell proliferation and apoptosis.
USP7
  • RNA pull-down and RNA immunoprecipitation assays to determine molecular interactions.

    • Main Results:

    • piR-hsa-23533 was found to be upregulated in HNSCC cells and tissues.
    • Overexpression of piR-hsa-23533 promoted proliferation and inhibited apoptosis in HNSCC cells, both in vitro and in vivo.
    • Silencing piR-hsa-23533 demonstrated opposite effects.
    • Mechanistically, piR-hsa-23533 binds to Ubiquitin-specific protease 7 (USP7), increasing its mRNA and protein expression.

    Conclusions:

    • piR-hsa-23533 plays a significant functional role in HNSCC.
    • The findings suggest piR-hsa-23533 as a potential therapeutic target for anti-HNSCC strategies.