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Solid phase speciation controls copper mobilisation from marine sediments by methanobactin.

Danielle D Rushworth1, Walter D C Schenkeveld2, Naresh Kumar2

  • 1Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria; Environmental Sciences, Copernicus Institute of Sustainable Development, Utrecht University, Utrecht, Netherlands.

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Chalkophores like methanobactin can mobilize copper from marine sediments, but only from specific copper sulfide phases. Sediment properties and chalkophore adsorption also limit copper bioavailability for methane-oxidizing bacteria.

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Area of Science:

  • Environmental microbiology
  • Biogeochemistry
  • Marine science

Background:

  • Marine environments store significant methane, a greenhouse gas.
  • Methanotrophs oxidize methane, limiting its atmospheric release.
  • Aerobic methanotrophs use copper-dependent enzymes, requiring efficient copper acquisition.

Purpose of the Study:

  • To determine if chalkophores can mobilize copper from sulfide-bearing marine sediments.
  • To investigate factors limiting chalkophore-mediated copper bioavailability.
  • To understand copper acquisition by methanotrophs in sulfidic marine environments.

Main Methods:

  • Kinetic batch experiments using methanobactin and marine sediments.
  • Characterization of solid-phase copper speciation using X-ray absorption spectroscopy and sequential extraction.
  • Investigation of chalkophore adsorption to marine sediments.

Main Results:

  • Copper mobilization by methanobactin was dependent on specific copper sulfide (CuSx) phases, not total copper content.
  • Chalkophore adsorption to sediments constrained copper mobilization, particularly in less compact sediments.
  • Methanobactin addition enhanced dissolved copper concentrations only in certain sediments.

Conclusions:

  • Chalkophores can mobilize copper from specific marine sediment types.
  • Copper bioavailability for methanotrophs is limited by solid-phase speciation and chalkophore adsorption.
  • Methanotrophic copper acquisition in sulfidic sediments is restricted to specific interfaces with mono-copper-sulfide phases.