JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Systematic screening of synthetic organochalcogen compounds with anticancer activity using human lung adenocarcinoma spheroids

  • 0Department of Biochemistry and Molecular Biology, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil; Laboratory of Cellular Biochemistry, Department of Biochemistry, Institute of Basic Health Sciences (ICBS), Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, RS, 90035-003, Brazil; National Institutes of Science and Technology-Translational Medicine (INCT-TM), Brazil.
Chemico-Biological Interactions +

|

May 12, 2024

|

May 12, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

New organochalcogen compounds show promise in treating lung adenocarcinoma. These novel agents demonstrated superior anticancer activity compared to cisplatin, offering a potential new therapeutic strategy for this deadly cancer.

Area Of Science

  • Medicinal Chemistry
  • Oncology
  • Drug Discovery

Background

  • Lung adenocarcinoma is a major cause of cancer mortality with limited treatment options.
  • Elevated oxidative stress markers correlate with aggressive cancer traits and poor patient outcomes.
  • Targeting aberrant redox biology presents an innovative strategy for lung adenocarcinoma intervention.

Purpose Of The Study

  • To screen novel synthetic organochalcogen compounds for anticancer activity.
  • To compare the efficacy of these compounds against cisplatin in lung adenocarcinoma models.
  • To explore the therapeutic potential of organochalcogens in lung adenocarcinoma treatment.

Main Methods

  • Established A549 human lung adenocarcinoma cell line for single tumor spheroid generation.
  • Determined optimal spheroid formation (4 days in vitro) and growth (20 days in vitro).
  • Conducted toxic dose-response curves for nine organochalcogen compounds and cisplatin after 24 and 48 hours incubation.

Main Results

  • At least two synthetic organochalcogen compounds exhibited significant anticancer activity.
  • These compounds outperformed cisplatin in terms of lower lethal dose 50 (LD50), larger drug activity area, and maximum effect amplitude.
  • In silico analysis predicted favorable physicochemical and ADME properties for selected compounds.

Conclusions

  • Synthetic organochalcogen compounds demonstrate superior efficacy compared to cisplatin for lung adenocarcinoma.
  • These compounds represent promising candidates for future therapeutic development.
  • This research opens new avenues for lung adenocarcinoma treatment by targeting redox biology.

Keywords: