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Polydopamine-Coated Solid Silica Nanoparticles Encapsulating IR-783 Dyes: Synthesis and NIR Fluorescent Cell Imaging
Yoshio Nakahara1, Haruki Nakabayashi1, Jun Miyazaki1
1Faculty of Systems Engineering, Wakayama University, 930 Sakae-dani, Wakayama 640-8510, Japan.
ACS Omega
|May 13, 2024
Summary
Researchers developed a simple method to create polydopamine-coated silica nanoparticles (s-SiO2@PDA NPs) that encapsulate near-infrared (NIR) dyes. These nanoparticles show potential for NIR fluorescent imaging in biological applications.
Area of Science:
- Nanomaterials Science
- Biomedical Engineering
- Analytical Chemistry
Background:
- Polydopamine (PDA) coatings offer versatile functionalization for nanoparticles.
- Near-infrared (NIR) fluorescent dyes are crucial for deep-tissue imaging due to minimal light scattering.
- Encapsulation strategies are needed to stabilize NIR dyes and improve their imaging performance.
Purpose of the Study:
- To develop a straightforward method for synthesizing polydopamine-coated solid silica nanoparticles (s-SiO2@PDA NPs).
- To investigate the encapsulation and fluorescence properties of anionic NIR dyes within s-SiO2@PDA NPs.
- To evaluate the stability and applicability of the developed nanoparticles for biological NIR imaging.
Main Methods:
- Synthesized solid silica nanoparticles (s-SiO2).
- Coated s-SiO2 with polydopamine (PDA) via physical adsorption under acidic aqueous conditions.
- Encapsulated anionic NIR fluorescent dyes, indocyanine green (ICG) and IR-783, within the s-SiO2@PDA NPs.
- Assessed dye encapsulation efficiency, fluorescence characteristics, and stability in a pseudobiological solution.
- Performed NIR fluorescent imaging of African green monkey kidney cells.
Main Results:
- Successfully synthesized s-SiO2@PDA NPs capable of encapsulating anionic NIR dyes.
- Observed fluorescence quenching for ICG, while IR-783 retained observable fluorescence after encapsulation.
- Demonstrated good photostability of encapsulated IR-783 in a pseudobiological solution over two weeks.
- Successfully utilized IR-783-loaded s-SiO2@PDA NPs for NIR fluorescent cell imaging.
Conclusions:
- A simple and efficient method for creating s-SiO2@PDA NPs for NIR dye encapsulation was established.
- The developed nanoparticles provide a stable platform for NIR fluorescent dyes, with potential for biological applications.
- The s-SiO2@PDA NPs encapsulating IR-783 are suitable for NIR fluorescent imaging of cells.
