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Related Experiment Video

Updated: Jun 26, 2025

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

Published on: August 16, 2024

The OptoReg system: a simple and inexpensive solution for regulating water oxygen.

Rasmus Ern¹, Fredrik Jutfelt^1,2

¹Department of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, 7034 Trondheim, Norway.

Conservation Physiology

|May 13, 2024

Summary

The OptoReg system offers a low-cost, precise method for controlling dissolved oxygen levels in water using readily available hardware and free software. This apparatus enables independent regulation of oxygen and nitrogen gas for aquatic experiments.

Keywords:

Cleware GmbH FireSting-O2 meter PyroScience RasmOx hyperoxia hypoxia kilopascal (kPa)oxygen partial pressure (PO2)oxygen regulator percent air saturation (% AS)

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Last Updated: Jun 26, 2025

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Published on: June 16, 2020

Area of Science:

Aquatic Science
Environmental Monitoring
Biotechnology

Background:

Accurate control of dissolved oxygen is crucial for aquatic research and environmental monitoring.
Existing methods for manipulating oxygen saturation can be complex and expensive.

Purpose of the Study:

To describe the development and implementation of a novel optocoupler-based regulation apparatus (OptoReg) for precise control of oxygen levels in water.
To provide a cost-effective and user-friendly solution for aquatic oxygen manipulation in laboratory settings.

Main Methods:

The OptoReg system utilizes an optocoupler box and electronic switch box to control solenoid valves for oxygen and nitrogen gas.
Hardware components connect to a computer via USB, controlled by free, graphical user interface software.
The system integrates with a 4-channel FireSting-O2 meter for real-time monitoring.

Main Results:

The OptoReg system demonstrated high precision and stability in regulating water oxygen levels.
Successful operation was shown during both static acclimation experiments and dynamic warming trials.
The setup allows for four independently controlled systems per computer and FireSting-O2 meter.

Conclusions:

The OptoReg apparatus provides an accessible and affordable method for precise dissolved oxygen control in aquatic research.
This system empowers laboratories to easily establish multiple independent oxygen regulation setups.
The described method facilitates advanced aquatic experimentation and environmental simulation.