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Updated: Jun 26, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Complete mitochondrial genome sequence and phylogenetic analysis of Tylopilus brunneirubens (Boletales,
Jie-Yu Huang1, Lei Tu2, Yan Lv1
1College of Life Science, Jiangxi Science and Technology Normal University, Nanchang, China.
Abstract:
Tylopilus brunneirubens is a common species in southern China. It is known for brown to dark brown pileus, white context turning reddish brown or rust brown when touched and distinct reticulation on the upper stem. However, little is known about its mitochondrial genome and its relationship with other boletes. Our analysis revealed that the mitochondrial genome of this species is a circular DNA molecule that spans 32,389 bp. It contains 15 core protein-coding genes, 24 transfer RNA genes, and two ribosomal RNA genes. The base composition of the mitochondrial genome is as follows: A (37.20%), C (11.32%), G (12.48%), and T (39.00%), with a GC content of 23.80%. Furthermore, a phylogenetic tree based on 24 mitochondrial genomes provided valuable insights into the phylogenetic relationships of Tylopilus brunneirubens with other boletes for the first time.
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