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Updated: Jun 26, 2025

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Published on: March 24, 2023

A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation.

Jennifer Moussa¹, Domingo Gargallo-Viola², Line Elnif Thomsen¹

¹Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark.

|May 13, 2024

Summary

Researchers screened 3500 compounds to find new ways to stop antibiotic resistance. Six promising compounds were identified that inhibit bacterial plasmid conjugation, offering hope against the spread of antimicrobial resistance.

Keywords:

Compound library Compound library screening Escherichia coli Plasmid conjugation

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Last Updated: Jun 26, 2025

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

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Published on: March 24, 2023

High-Resolution Comparison of Bacterial Conjugation Frequencies

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Published on: January 10, 2019

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

Area of Science:

Microbiology
Molecular Biology
Drug Discovery

Background:

Antibiotic resistance is a major global health threat, driven by plasmid conjugation in bacteria.
New strategies are urgently needed to combat the spread of antimicrobial resistance.

Purpose of the Study:

To develop and apply a high-throughput screening method to identify compounds that inhibit plasmid conjugation.
To discover novel inhibitors of bacterial conjugation for potential therapeutic use.

Main Methods:

A library of 3500 compounds was screened using *Escherichia coli* donor and recipient strains.
High-throughput screening involved spotting bacterial cultures treated with compounds onto agar plates for visual assessment.
Compounds showing an effect on conjugation were retested for confirmation.

Main Results:

Six compounds were identified that demonstrated an ability to inhibit or reduce plasmid conjugation.
These compounds represent potential leads for developing new antimicrobial resistance strategies.

Conclusions:

The developed high-throughput screening method is effective in identifying conjugation inhibitors.
The identified compounds warrant further investigation as potential agents to combat antibiotic resistance.