Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Znrf2-mediated ST2L degradation limits IL-33/ST2L anti-fibrotic signaling and promotes post-infarction cardiac fibrosis.

Cellular signalling·2026
Same author

Major histocompatibility complex II serves as a prognostic biomarker in resectable pulmonary sarcomatoid carcinoma: development of a prediction model.

Translational lung cancer research·2026
Same author

Knowledge, attitudes and practices towards cardiac rehabilitation among patients with heart failure.

BMC public health·2026
Same author

Outcomes of simplified left atrial appendage occlusion using the WATCHMAN FLX device: the ROSE-FLX study.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
Same author

Transcriptome and plasma proteome analyses identify susceptibility genes and proteins for alcohol-related hepatocellular carcinoma.

Human genomics·2026
Same author

Clinical outcomes and tumor immune microenvironment in SMARCA4-Deficient NSCLC: A Real-World retrospective study.

Lung cancer (Amsterdam, Netherlands)·2026

Related Experiment Video

Updated: Jun 26, 2025

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.3K

Apolipoprotein B Secretion Assay from Primary Hepatocytes.

Yawei Wang1,2, Xin Li1,3, Runze Huang1,3

  • 1State Key Laboratory of Membrane Biology, Peking University, Beijing, China.

Bio-Protocol
|May 13, 2024
PubMed
Summary

This study presents a simple method to measure Apolipoprotein B (APOB) lipoprotein secretion using mouse liver cells. This technique aids in understanding lipid metabolism and cardiovascular disease mechanisms.

Keywords:
Cardiovascular diseasesHyperlipidemiaLipoprotein secretionPrimary hepatocytesWestern blot

More Related Videos

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

11.4K
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
11:34

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

2.2K

Related Experiment Videos

Last Updated: Jun 26, 2025

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.3K
"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

11.4K
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
11:34

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

2.2K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cardiovascular Research

Background:

  • Apolipoprotein B (APOB) is crucial for atherogenic lipoproteins, contributing to cardiovascular diseases (CVDs).
  • Lipoprotein secretion is regulated by endocytic and exocytic pathways, with the latter being less understood.
  • Understanding APOB lipoprotein secretion is vital for addressing CVDs.

Purpose of the Study:

  • To provide a straightforward protocol for detecting Apolipoprotein B (APOB) and lipoprotein secretion.
  • To establish a method for analyzing the cellular lipid metabolism and lipoprotein secretion process.
  • To facilitate research into the mechanisms underlying lipoprotein-related diseases.

Main Methods:

  • Primary mouse hepatocyte isolation and cell culture.
  • Utilizing immunoblotting for sensitive and specific detection of APOB.
  • Semi-quantitative analysis of lipoprotein secretion.

Main Results:

  • A reliable pipeline for isolating and culturing primary mouse hepatocytes was established.
  • A procedure for tracking APOB-containing lipoprotein secretion was successfully implemented.
  • A rapid and sensitive immunoblotting assay for APOB detection was developed.

Conclusions:

  • The described method offers a simple, effective tool for studying APOB lipoprotein secretion.
  • This protocol aids in exploring cellular lipid metabolism and lipoprotein-related disease mechanisms.
  • The technique is valuable for advancing research in cardiovascular disease.