Related Experiment Video
Updated: Jun 26, 2025
17:41
Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil
Published on: January 4, 2014
23.2K
DENV-2 Outbreak Associated With Cosmopolitan Genotype Emergence in Western Brazilian Amazon
Jessiane Rodrigues Ribeiro1,2, Tárcio Peixoto Roca2,3, Gleense Dos Santos Cartonilho1,2,4
1Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia-Fiocruz Rondônia (FIOCRUZ/RO), Porto Velho, Brazil.
Bioinformatics and Biology Insights
|May 13, 2024
Summary
Dengue virus (DENV) serotypes DENV-1 and DENV-2 are co-circulating in the Western Brazilian Amazon. The study identified the DENV-2 Cosmopolitan genotype in Rondônia, highlighting its rapid spread.
Area of Science:
- Medical Entomology
- Virology
- Public Health
Background:
- Dengue virus (DENV) poses a significant public health threat in the Western Brazilian Amazon, causing the most arbovirus infections and deaths.
- Four DENV serotypes have previously circulated in the region, necessitating ongoing surveillance.
Purpose of the Study:
- To molecularly characterize arboviruses in febrile patients in the Western Brazilian Amazon.
- To identify circulating DENV serotypes and genotypes, including the detection of novel introductions.
Main Methods:
- Analysis of 841 samples from febrile patients (2021-2023) using a commercial arbovirus detection kit.
- Sanger sequencing of positive samples to determine DENV serotypes and genotypes.
Main Results:
- 162 samples tested positive; 12 were DENV-1 and 150 were DENV-2, confirming co-circulation.
- Sequencing identified DENV-1 genotype V (5 samples) and DENV-2 Cosmopolitan genotype-lineage 5 (46 samples).
Conclusions:
- The DENV-2 Cosmopolitan genotype was identified for the first time in Rondônia, Western Brazilian Amazon.
- Evidence suggests rapid dispersion of the identified DENV-2 genotype within the region.
