Investigation of GNB1 derivative circular RNAs hsa_circ_0009361 and hsa_circ_0009362 expressions in colorectal cancer...

Area of Science:

• Molecular Oncology
• Genomics
• Biomarker Discovery

Background:

• Circular RNAs (circRNAs) play a role in colorectal cancer (CRC) development.
• circRNAs are emerging as diagnostic and prognostic biomarkers for CRC.
• Investigating circRNAs derived from the GNB1 gene (hsa_circ_0009361 and hsa_circ_0009362) is crucial for understanding CRC.

Purpose of the Study:

• To investigate the expression levels of hsa_circ_0009361 and hsa_circ_0009362 in colorectal cancer (CRC) patients.
• To explore the relationship between these circRNAs and clinicopathological features of CRC.
• To evaluate the diagnostic potential of hsa_circ_0009361 and hsa_circ_0009362 in CRC.

Main Methods:

• Quantitative real-time PCR (qRT-PCR) was used to measure circRNA expression in 45 CRC and adjacent control tissues.
• Receiver operating characteristic (ROC) curve analysis was performed to assess diagnostic power.
• Correlation analysis was conducted to evaluate the association between circRNA expression levels.

Main Results:

• Expression levels of hsa_circ_0009361 and hsa_circ_0009362 were significantly downregulated in CRC tissues compared to controls.
• CircRNA expression correlated with lymph vascular invasion in CRC patients (P<0.001).
• ROC curve analysis indicated these circRNAs are potential diagnostic biomarkers for CRC, with a positive correlation between their expression levels (P<0.0001).

Conclusions:

• hsa_circ_0009361 and hsa_circ_0009362 may serve as valuable diagnostic biomarkers for colorectal cancer (CRC).
• The downregulation of these circRNAs is associated with specific clinicopathological features of CRC.