Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenic CD8+ T cells target K71+ Henle's layer by forming cytolytic immune synapses in Alopecia Areata.

Research square·2026
Same author

CXCR4 coordinates adhesion, migration, and development of human NK cells.

bioRxiv : the preprint server for biology·2026
Same author

Autosomal dominant CDC45 deficiency with allelic expression bias causes a novel genetic disease of the immune system.

bioRxiv : the preprint server for biology·2026
Same author

Fibroblast activation protein promotes natural killer cell invasion and tumor infiltration.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Systematic analysis of cellular cross-talk reveals a role for SEMA6D-TREM2 regulating microglial function in Alzheimer's disease.

Science translational medicine·2025
Same author

Joint representation and visualization of derailed cell states with Decipher.

Genome biology·2025
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
Same journal

The nucleus-vacuole junction at a glance.

Journal of cell science·2026
Same journal

Loss of INPP5E affects photoreceptor outer segment membrane biogenesis in iPSC-derived human retinal organoids.

Journal of cell science·2026
Same journal

Brinker regulates reciprocal outcomes of BMP signal between stem cells and differentiating cells.

Journal of cell science·2026
Same journal

Primary cilium disassembly - from mechanisms to roles in physiology and disease.

Journal of cell science·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2025

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

5.9K

cellPLATO - an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data.

Michael J Shannon1, Shira E Eisman1, Alan R Lowe2

  • 1Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University Medical Center, NYC, NY 10032, USA.

Journal of Cell Science
|May 13, 2024
PubMed
Summary
This summary is machine-generated.

A new software, cellPLATO, analyzes cell behavior from microscopy data. It reveals Interleukin-15 (IL-15) enhances natural killer (NK) cell migration plasticity on ICAM-1 and VCAM-1.

Keywords:
Cell migrationCell morphologyData-driven analysisIL-15IntegrinsNK cellsPhenomicsUnsupervised machine learning

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K

Related Experiment Videos

Last Updated: Jun 26, 2025

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

5.9K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K

Area of Science:

  • * Computational Biology
  • * Cell Biology
  • * Immunology

Background:

  • * Microscopy generates large, complex datasets requiring advanced analysis tools.
  • * Phenotypic analysis of cell behavior is crucial for understanding biological processes.
  • * Existing tools may not adequately capture dynamic cell morphology and motility patterns.

Purpose of the Study:

  • * To introduce cellPLATO, a Python-based software for analyzing cell behavior from microscopy.
  • * To classify cell behaviors based on morphology and motility features.
  • * To investigate the role of Interleukin-15 (IL-15) in human natural killer (NK) cell migration.

Main Methods:

  • * CellPLATO software segments and tracks cells, extracting morphological and motility features per timepoint.
  • * Dimensionality reduction and clustering are used to segregate cells into behavioral subtypes.
  • * Similarity analysis groups behavioral sequences into discrete trajectories with assigned IDs.
  • * Human NK cells were cultured and their migration on ICAM-1 and VCAM-1 was analyzed with and without IL-15.

Main Results:

  • * CellPLATO identified eight distinct NK cell behavioral subsets based on single timepoint features.
  • * Four distinct NK cell migration trajectories were identified based on sequences of behaviors.
  • * IL-15 treatment significantly increased the plasticity between different NK cell migration behaviors.
  • * Migration patterns differed significantly between ICAM-1 and VCAM-1 substrates.

Conclusions:

  • * CellPLATO is an effective tool for analyzing complex cell behavior in microscopy data.
  • * IL-15 enhances NK cell adaptability in migration patterns.
  • * Integrin ligands ICAM-1 and VCAM-1 differentially regulate NK cell migration dynamics.