Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

1.3K
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
1.3K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

1.2K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
1.2K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

870
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
870

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ATP-responsive injectable DNA hybrid hydrogels for regulating the "on-demand release" of fibroblast growth factor 21 cytokines to facilitate muscle regeneration.

International journal of biological macromolecules·2026
Same author

Clinical efficacy of linezolid in the treatment of tuberculous meningitis: a retrospective analysis and literature review.

BMC infectious diseases·2025
Same author

Oligoadenine Strand Functionalized Polyacrylamide Hydrogel Film Exhibiting pH-Triggered High-Degree Inverse Shape Deformations.

Chembiochem : a European journal of chemical biology·2024
Same author

Proton-Mediated Dynamic Nestling of DNA Payloads Within Size-Matched MOFs Nanochannels for Smart Intracellular Delivery.

Small (Weinheim an der Bergstrasse, Germany)·2024
Same author

Smart Free-Standing Bilayer Polyacrylamide/DNA Hybrid Hydrogel Film-Based Sensing System Using Changes in Bending Angles as a Visual Signal Readout.

Analytical chemistry·2024
Same author

DNA Cryogels with Anisotropic Mechanical and Responsive Properties for Specific Cell Capture and Release.

Journal of the American Chemical Society·2024

Related Experiment Video

Updated: May 3, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

9.9K

Dual stimuli-responsive upconversion nanoparticle-poly-N-isopropylacrylamide/DNA core-shell microgels.

Qi Gao1, Xiaowen Wang1, Shanjin Hu1

  • 1Research Center for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, P. R. China; Smart Sensing Interdisciplinary Science Center, School of Materials Science and Engineering, Nankai University, Tianjin 300350, P. R. China. weiweiguo@nankai.edu.cn.

Soft Matter
|May 13, 2024
PubMed
Summary

New stimuli-responsive microgels combine upconversion nanoparticles (UCNPs) and DNA for smart drug delivery. These UCNP-pNIPAM/DNA microgels offer tunable sizes and programmable functions, controlled by light and temperature.

More Related Videos

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

9.8K
Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

986

Related Experiment Videos

Last Updated: May 3, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

9.9K
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

9.8K
Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

986

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Upconversion nanoparticles (UCNPs) offer unique near-infrared (NIR) responsive properties.
  • Poly-N-isopropylacrylamide (pNIPAM) is a well-known thermosensitive polymer.
  • DNA nanotechnology enables precise control over molecular functions.

Purpose of the Study:

  • To prepare stimuli-responsive core-shell microgels integrating UCNPs, pNIPAM, and DNA.
  • To achieve tunable microgel sizes and programmable functionalities.
  • To enable smart regulation of DNA activity using external stimuli.

Main Methods:

  • Synthesis of UCNP-pNIPAM/DNA core-shell microgels.
  • Characterization of microgel size and morphology.
  • Assessment of DNA loading efficiency and activity.
  • Evaluation of NIR and temperature-triggered DNA regulation.

Main Results:

  • Successfully prepared UCNP-pNIPAM/DNA core-shell microgels with tunable sizes.
  • Achieved high DNA activity and loading efficiency within the microgels.
  • Demonstrated simultaneous regulation of DNA activity by NIR illumination and temperature.
  • UCNP cores provide NIR responsiveness, while pNIPAM shells offer thermosensitivity.

Conclusions:

  • The developed microgels exhibit excellent stimuli-responsive behavior for controlled DNA release and activity.
  • These smart microgels hold promise for advanced applications in drug delivery and diagnostics.
  • The combination of UCNPs, pNIPAM, and DNA offers a versatile platform for functional nanomaterials.