Quantitative analysis of therapeutic peptides by CZE using multiple sample injection in hydrodynamically closed...

Area of Science:

• Analytical Chemistry
• Pharmaceutical Science

Background:

• Therapeutic peptides like triptorelin and lanreotide are vital in modern medicine.
• Developing precise analytical methods is crucial for their quality control and clinical use.

Purpose of the Study:

• To develop and validate novel capillary electrophoresis (CE) methods for determining triptorelin and lanreotide.
• To establish the first CE-based method for lanreotide and the first hydrodynamically closed system (HCS) CE method for triptorelin.

Main Methods:

• Capillary zone electrophoresis (CE) in a hydrodynamically closed system (HCS) with ultraviolet detection.
• Repeated injection sample introduction technique.
• Optimization of background electrolytes using formic acid and methyl-hydroxyethyl cellulose.

Main Results:

• Achieved maximal separation efficiency and signal intensity.
• Demonstrated excellent calibration curves (r² > 0.99), low limits of detection (0.25-0.5 µg/mL), and acceptable precision and accuracy.
• Successfully quantified triptorelin in a drug formulation and spiked urine samples.

Conclusions:

• The developed CE methods are highly efficient, accurate, and precise for analyzing triptorelin and lanreotide.
• These methods show superior applicability for quality control and clinical investigations of therapeutic peptides.