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Related Experiment Video

Updated: Jun 26, 2025

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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Transcriptome analysis of long non-coding RNA associated with embryonic muscle development in chickens.

Y Yuan1, W Duan1, N Yang1

  • 1Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

British Poultry Science
|May 13, 2024
PubMed
Summary

This study investigated long non-coding RNAs (lncRNAs) in chicken muscle development. It identified key lncRNA-mRNA interactions, highlighting MSTRG.12395.2-FGFBP2 and MSTRG.18590.6-FMOD as crucial for embryonic muscle growth.

Keywords:
ChickenembryoniclncRNAmuscle fibretarget gene

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Area of Science:

  • Animal Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Skeletal muscle development in chickens is critical for carcass quality and is largely determined during the embryonic period.
  • Beijing-You (BY) and Cornish (CN) chickens exhibit distinct growth rates and body types, making them valuable models for studying muscle development.

Purpose of the Study:

  • To explore the role of long non-coding RNAs (lncRNAs) in chicken muscle development across different embryonic stages.
  • To identify differentially expressed lncRNAs and messenger RNAs (mRNAs) between BY and CN chicken breeds during embryonic development.

Main Methods:

  • RNA sequencing (RNA-seq) was performed on pectoral muscle tissues from BY and CN chickens at embryonic days 11, 13, 15, 17, and 1-day-old.
  • Systematic analysis of lncRNAs and mRNAs was conducted to identify differentially expressed transcripts (DETs).
  • Correlation analysis was used to predict lncRNA-mRNA interactions, with key pairs validated experimentally.

Main Results:

  • A total of 4,104 DETs, including 2,359 lncRNAs and 1,745 mRNAs, were identified across the five developmental stages.
  • The greatest difference in gene regulation between BY and CN breeds occurred at embryonic day 17, with 1,658 lncRNAs and 1,016 mRNAs identified as differentially expressed.
  • Forty-three cis interaction pairs of lncRNA-mRNA related to muscle development were predicted, and MSTRG.12395.2-FGFBP2 and MSTRG.18590.6-FMOD were validated as significantly upregulated in CN chickens at ED11.

Conclusions:

  • lncRNAs play a significant role in regulating embryonic muscle development in chickens.
  • Specific lncRNA-mRNA interactions, such as MSTRG.12395.2-FGFBP2-FGFBP2 and MSTRG.18590.6-FMOD, are potential key regulators of muscle growth potential in chickens.