The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Hepatocellular carcinoma (HCC) diagnosis can be improved using long non-coding RNAs (lncRNAs). CASC2 shows higher accuracy and sensitivity than MALAT1 and AFP for detecting HCC, especially in patients with Hepatitis C Virus (HCV).
Area Of Science
- Oncology
- Molecular Biology
- Biomarker Discovery
Background
- Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality globally.
- Lack of early diagnostic tools necessitates novel biomarker research.
- Long non-coding RNAs (lncRNAs) are implicated in various pathologies, including HCC.
Purpose Of The Study
- To evaluate the diagnostic potential of lncRNAs MALAT1 and CASC2 in HCC.
- To compare the efficacy of MALAT1 and CASC2 against the established biomarker AFP.
Main Methods
- A case-control study involving 89 participants (HCC/HCV, HCV, and healthy controls).
- Radiological examinations, complete blood count (CBC), and liver function tests were performed.
- Hepatitis C Virus (HCV) RNA quantification and lncRNA expression (MALAT1, CASC2) via qRT-PCR.
Main Results
- Significant differences in liver function tests were observed between groups, with higher levels in HCC/HCV patients.
- Serum MALAT1 was upregulated in HCV and HCC/HCV groups compared to controls.
- Serum CASC2 was upregulated in HCV but downregulated in HCC patients; CASC2 demonstrated higher diagnostic accuracy (94.6%) and sensitivity (97.2%) than AFP and MALAT1.
Conclusions
- CASC2 shows significant promise as a diagnostic biomarker for HCC.
- CASC2 outperforms MALAT1 and AFP in accuracy and sensitivity for HCC diagnosis, particularly in the context of HCV co-infection.
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