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Bead-beating assay during synovial fluid DNA extraction improves real-time PCR accuracy for periprosthetic joint
Yuta Hieda1, Hyonmin Choe1, Hiroyuki Ike1
1Department of Orthopaedic Surgery, Yokohama City University, Yokohama City, Kanagawa, Japan.
Abstract:
Polymerase chain reaction (PCR)-based genetic diagnosis is a rapid and sensitive method to diagnose periprosthetic joint infection (PJI). DNA extraction using bead beating is an effective method for collecting bacterial genes in Gram-positive bacteria. We compared the detection accuracy between the conventional and bead-beating DNA extraction assay. The detection rate improved from 86.7% using the conventional method to 95.6% using the bead-beating. Our results suggest that bead-beating during DNA extraction can improve the accuracy of PCR-based genetic diagnosis of PJI.
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