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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

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No Ranaviral DNA Found in Australian Freshwater Turtles, 2014-19, Despite Previous Serologic Evidence.

Wytamma Wirth¹, Lin Schwarzkopf², Jason Schaffer³

¹College of Public Health, Medical and Veterinary Sciences, 1 James Cook Drive, James Cook University, Townsville, Queensland 4814, Australia.

Journal of Wildlife Diseases

|May 14, 2024

Summary

This study surveyed wild Australian freshwater turtles for ranaviruses, finding no viral DNA despite historical evidence of antibodies. Further research is needed to understand ranaviral presence in these reptiles.

Keywords:

Australia epidemiology freshwater turtles ranavirus survey

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Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

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Published on: November 10, 2018

Area of Science:

Herpetology
Virology
Wildlife Disease Ecology

Background:

Ranaviruses infect ectothermic vertebrates, with turtles being commonly affected reptiles.
No surveys have assessed wild ranaviral infections in Pleurodira turtles, a suborder with overlapping populations and distributions with ranaviruses, particularly in Australia.

Purpose of the Study:

To investigate the prevalence of ranaviral infection in wild Australian freshwater turtles of the suborder Pleurodira.
To compare molecular findings with historical serologic data indicating ranaviral antibodies in the region.

Main Methods:

Analysis of 397 pooled blood samples from six Australian freshwater turtle species.
Samples were collected from five distinct sites in northern Australia between 2014 and 2019.
Ranaviral DNA detection using molecular assays.

Main Results:

No ranaviral DNA was detected in any of the assayed turtle blood samples.
This contrasts with historical serologic surveys that identified anti-ranaviral antibodies in the region.

Conclusions:

The absence of detectable ranaviral DNA suggests low prevalence during the sampling period or potential biases in sampling methods.
Further investigation is warranted to reconcile molecular and serologic data and understand ranaviral dynamics in Australian Pleurodira turtles.