Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

596
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
596
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

161
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
161
Flame Photometry: Lab01:16

Flame Photometry: Lab

243
In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
243
Fluid Mosaic Model01:19

Fluid Mosaic Model

11.6K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
11.6K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

378
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
378
Flame Photometry: Overview01:02

Flame Photometry: Overview

564
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
564

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiological and hormonal stress response during blood donation in healthy voluntary donors.

Asian journal of transfusion science·2026
Same author

A tertiary care interventional study on autologous platelet-rich plasma regenerative therapy for Grade III knee osteoarthritis.

Asian journal of transfusion science·2026
Same author

A study to assess and grade the donor adverse events using the severity grading tool of hemovigilance program of India.

Asian journal of transfusion science·2026
Same author

Adverse Reactions to Blood Transfusion in Pediatric Cardiac Surgery: A Retrospective Cohort Study at a Single Center.

Annals of cardiac anaesthesia·2026
Same author

Transfusion knowledge online: A Wikipedia deep dive.

Vox sanguinis·2026
Same author

A follow-up study of blood donors with vasovagal reaction - Three years' experience.

Asian journal of transfusion science·2025

Related Experiment Video

Updated: Jun 26, 2025

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

12.8K

Amber coloured plasma-Case for illustration.

Amita Radhakrishnan Nair1, Baby Saritha Gopalakrishnan1, Debasish Gupta1

  • 1Department of Transfusion Medicine, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.

Transfusion Medicine (Oxford, England)
|May 14, 2024
PubMed
Summary

High coffee consumption can cause orange-colored plasma in blood donors. This is the first report linking quinic acid from coffee to blood product discoloration, necessitating further safety studies.

Keywords:
amber coloured plasmacoffee intakeliquid chromatographyplasma discolourationspectrophotometry

More Related Videos

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry
07:17

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry

Published on: August 1, 2017

12.6K
An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation
08:36

An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation

Published on: November 3, 2016

9.9K

Related Experiment Videos

Last Updated: Jun 26, 2025

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

12.8K
Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry
07:17

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry

Published on: August 1, 2017

12.6K
An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation
08:36

An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation

Published on: November 3, 2016

9.9K

Area of Science:

  • Transfusion Medicine
  • Analytical Chemistry
  • Biochemistry

Background:

  • Abnormal plasma coloration is uncommon, with causes including hemolysis, diet, or medications.
  • Identifying the cause of plasma discoloration is crucial for donor advice and blood product management.

Observation:

  • Two cases of orange-colored plasma in regular blood donors were investigated.
  • Standard tests ruled out hemolysis as the cause of discoloration.

Findings:

  • Spectrophotometry revealed a unique peak in the discolored plasma samples.
  • Liquid Chromatography-Mass Spectrometry (LC-MS) identified quinic acid residues in the orange plasma, linked to high coffee intake.

Implications:

  • This is the first reported case of quinic acid causing blood product discoloration.
  • Further research is needed to confirm the safety of transfusing blood products from donors with quinic acid-related plasma discoloration.