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[Evaluation of ototoxic effect of micronomicin by intravenous drip infusion]
Abstract:
Ototoxic effect of micronomicin (MCR) in intravenous drip administration was investigated in guinea pigs (300--400 g) receiving MCR for 30 days at dose of 50 and 100 mg/kg, respectively. MCR was dissolved in physiologic saline and 1.5 ml of the solution was infused through polyethylene tube into the left external jugular vein with Perista mini-pump for 60 minutes every day after measurement of body weight. Auditory impairment was monitored by pinna reflex audiometry. Pinna reflex loss was not detected in any animal in frequency range (0.5 to 20 kHz). Cochlear hair cell damage generally was of mild degree. Two out of 6 animals treated with MCR 50 mg/kg (Table 1) showed outer hair cell loss confined to unilateral basal end and posterior 3/4 of the first turn, respectively. Outer hair cell loss was noticed in 4 of 9 animals receiving MCR 100 mg/kg (Table 2); much less extensive in 3 animals, unsymmetrical slightly extensive in remaining 1. Unilateral circumscribed loss of inner hair cells was noticed at lower part of the hook in the latter one. Vestibular hair cell loss was scattered and less extensive and occurred in 4 of the 6 50 mg/kg given animals and in 7 of the 9 100 mg/kg ones. Comparison in incidence and extension of the outer hair cell loss of the cochlea (Tables 3, 4) in the present study and previous ones on the ototoxic effect of MCR in intramuscular and intravenous administration suggests that there was no distinct difference in enhancement of the ototoxic effect in the intravenous drip administration.
Insights
Intravenous drip administration of micronomicin (MCR) showed mild ototoxic effects in guinea pigs, with no significant difference compared to other administration routes. Hair cell damage was generally mild.
Area of Science:
- Ototoxicology
- Pharmacology
- Auditory Science
Background:
- Micronomicin (MCR) is an aminoglycoside antibiotic.
- Aminoglycosides can cause ototoxicity, affecting hearing and balance.
- Intravenous administration routes require careful evaluation for toxicity.
Purpose of the Study:
- To investigate the ototoxic effects of micronomicin (MCR) when administered via intravenous drip.
- To compare the ototoxicity of intravenous drip MCR with previously studied intramuscular and intravenous administrations.
- To assess auditory function and cochlear/vestibular hair cell damage in guinea pigs.
Main Methods:
- Guinea pigs received MCR (50 and 100 mg/kg) intravenously for 30 days.
- Auditory function was monitored using pinna reflex audiometry.
- Cochlear and vestibular hair cells were examined for damage post-treatment.
Main Results:
- No auditory impairment was detected via pinna reflex audiometry across the tested frequency range (0.5-20 kHz).
- Cochlear outer hair cell loss was mild, observed in 2/6 animals at 50 mg/kg and 4/9 animals at 100 mg/kg.
- Vestibular hair cell loss was scattered and less extensive, occurring in 4/6 animals (50 mg/kg) and 7/9 animals (100 mg/kg).
Conclusions:
- Intravenous drip administration of MCR resulted in generally mild ototoxic effects in guinea pigs.
- The incidence and extent of cochlear hair cell loss showed no distinct difference compared to previous studies of MCR's intramuscular and intravenous administration.
- Intravenous drip administration of MCR does not appear to enhance its ototoxic effect compared to other routes.