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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Related Experiment Video

Updated: Jun 26, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering.

Se Ra Lim1, Sang Jun Lee1

  • 1Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea.

Journal of Agricultural and Food Chemistry
|May 14, 2024
PubMed
Summary
This summary is machine-generated.

Multiplex genome editing enables simultaneous genetic modifications in microbes, accelerating the development of engineered strains. This review explores its applications, AI integration, and future impact on the agriculture and food industries.

Keywords:
Cas nucleasebase editorguide RNAmicrobial productionmultiplex genome editing

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Area of Science:

  • Synthetic Biology and Genetic Engineering
  • Microbial Biotechnology
  • Bioinformatics and Computational Biology

Background:

  • Genome editing is essential for developing desired phenotypes across diverse species.
  • CRISPR-Cas technology allows precise DNA sequence modification via guide RNA (gRNA).
  • Simultaneous editing of multiple targets accelerates the creation of engineered microbial strains.

Purpose of the Study:

  • To review multiplex genome editing resolution and its application in engineering bacteria and yeast.
  • To examine the role of artificial intelligence in advancing microbial genome editing.
  • To provide perspectives on the future of multiplex genome editing in agriculture and food production.

Main Methods:

  • Review of current literature on multiplex genome editing techniques and their efficiency.
  • Analysis of case studies involving engineered bacteria and yeast.
  • Exploration of AI algorithms and tools applicable to microbial genome engineering.

Main Results:

  • Multiplex genome editing significantly reduces time and cost for strain development.
  • Simultaneous multi-target editing enables the introduction of complex traits into microorganisms.
  • AI integration shows promise for optimizing gRNA design and predicting editing outcomes.

Conclusions:

  • Multiplex genome editing is a powerful tool for microbial engineering.
  • AI advancements are poised to further enhance the precision and efficiency of these technologies.
  • The agriculture and food industries stand to benefit significantly from future developments in multiplex genome editing.