Genome-wide identification and expression analysis of the Eriobotrya japonica TIFY gene family reveals its functional diversity under abiotic stress conditions

Xulin Li ¹, Ke Wen ², Ling Zhu ¹

⁰Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, China.

Bmc Genomics +

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May 14, 2024

View abstract on PubMed

Summary

Loquat EjTIFY genes were identified and analyzed for their roles in plant stress responses. This research provides a foundation for improving loquat salt stress resistance through targeted breeding strategies.

Area Of Science

Plant molecular biology
Genomics
Biotechnology

Background

Plant-specific TIFY proteins are crucial for plant adversity responses.
Loquat (Eriobotrya japonica) TIFY gene family studies were previously lacking.
Genomic and functional analyses of EjTIFY genes are needed.

Purpose Of The Study

To bioinformatically analyze the loquat EjTIFY gene family.
To investigate the chromosomal localization, gene structure, and expression profiles of EjTIFY genes under abiotic stress.
To provide a theoretical basis for understanding EjTIFY functions and enhancing loquat stress resistance.

Main Methods

Phylogenetic tree construction
Chromosomal localization analysis
Gene structure analysis
Abiotic stress expression profiling (RNA-seq, qRT-PCR)
Ka/Ks ratio analysis

Main Results

Twenty-six EjTIFY genes were identified and classified into four subfamilies.
EjTIFY genes showed varied chromosomal distribution and duplication patterns.
Promoter regions contained numerous abiotic stress cis-elements.
EjTIFY genes exhibited differential expression under gibberellin, low-temperature, high-temperature, and salt stresses.
Specific EjJAZ genes (EjJAZ2, EjJAZ4, EjJAZ9, EjJAZ15) were identified as key players in stress response pathways, particularly the jasmonic acid (JA) pathway.

Conclusions

The study characterized the EjTIFY gene family in loquat.
Findings offer insights into the functional roles of EjTIFY genes in stress tolerance.
Results support future research on loquat breeding for enhanced salt stress resistance.

Keywords:

TIFY gene family Abiotic stress Evolution Expression profile Loquat