Identification of metabolic components of carotid plaque in high-risk patients utilizing liquid chromatography-tandem mass spectrometry
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View abstract on PubMed
Summary
This summary is machine-generated.This study identified key metabolic biomarkers in carotid plaques, offering new insights for diagnosing stroke risk and developing targeted treatments for carotid atherosclerosis.
Area Of Science
- Biochemistry
- Vascular Biology
- Metabolomics
Background
- Carotid atherosclerosis is a progressive vascular disease.
- Stroke is a severe complication of advanced carotid atherosclerosis.
- Lack of reliable biomarkers hinders early diagnosis and treatment of high-risk individuals.
Purpose Of The Study
- To identify metabolic markers in carotid plaques associated with stroke risk.
- To explore potential targets for pharmacological interventions in carotid atherosclerosis.
Main Methods
- Untargeted metabolomics using liquid chromatography-tandem mass spectrometry on carotid intima and plaque tissues from 20 patients.
- Multivariate and univariate statistical analyses were employed.
Main Results
- 154 metabolites were significantly altered in carotid plaques compared to thickened intima.
- Key biomarkers including N-(cyclopropylmethyl)-N'-phenylurea, 9(S)-HOTrE, and l-thyroxine were identified.
- Disrupted purine, nucleotide, amino acid, glutathione, and vitamin metabolism pathways were implicated in plaque progression.
Conclusions
- Identified novel biomarkers for stroke-prone carotid plaques.
- Elucidated metabolic mechanisms underlying carotid atherosclerosis progression.
- Provides a basis for precise prevention and targeted therapies for carotid atherosclerosis.
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