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Related Experiment Video

Updated: Jun 26, 2025

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV
07:02

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV

Published on: September 14, 2010

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Single-Cell Transcriptomic Analysis of Kaposi Sarcoma.

D A Rauch, P Valiño Ramos, M Khanfar

    Biorxiv : the Preprint Server for Biology
    |May 15, 2024
    PubMed
    Summary
    This summary is machine-generated.

    Single cell analysis reveals two types of Kaposi Sarcoma (KS)-associated herpesvirus 8 (KSHV)-infected cells in tumors, including lymphatic endothelial and vascular tip cells. This research identifies novel biomarkers and provides insights into KS tumor composition and therapeutic responses.

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    Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells
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    Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

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    Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV
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    Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells
    00:06

    Quantitative Fluorescence In Situ Hybridization FISH and Immunofluorescence IF of Specific Gene Products in KSHV-Infected Cells

    Published on: August 27, 2019

    12.5K

    Area of Science:

    • Oncology
    • Virology
    • Genomics

    Background:

    • Kaposi Sarcoma (KS) is a KSHV-driven malignancy affecting various organs, particularly in immunosuppressed individuals.
    • Tumor histology shows a mix of spindle cells, vascular structures, and immune cells, necessitating deeper cellular characterization.
    • Understanding the cellular landscape of KS is crucial for developing targeted therapies.

    Purpose of the Study:

    • To identify and characterize KSHV-infected cell populations within KS tumors using single-cell RNA sequencing.
    • To discover novel cellular biomarkers associated with KSHV infection in KS.
    • To investigate the impact of therapeutic interventions on KS tumor cellular composition.

    Main Methods:

    • Single-cell RNA sequencing of skin and blood samples from twelve KS subjects.
    • Analysis of gene expression patterns to differentiate cell types and identify KSHV-infected cells.
    • Correlation of KSHV-infected cell populations with clinical parameters and therapeutic interventions.

    Main Results:

    • Two distinct KSHV-infected cell populations identified: proliferative lymphatic endothelial cells and angiogenic vascular tip cells.
    • Both infected cell populations express lytic and latent KSHV genes.
    • Novel biomarkers, including SCN9A, identified in KSHV-infected cells.
    • KSHV-positive tumor cells constitute ~6% in HIV-associated KS, inversely correlating with immune cells.
    • Expanded T-cell receptor clones observed in KS tumors and blood.
    • Therapeutic interventions (antiretroviral therapy, immunotherapy) altered tumor cellular composition.

    Conclusions:

    • Single-cell analysis is a feasible approach for dissecting KS tumor heterogeneity.
    • Identification of specific infected cell types and biomarkers offers prognostic and predictive potential.
    • Understanding cellular dynamics in KS is key to monitoring treatment response and developing novel therapies.