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Updated: Jun 28, 2026

Fabrication of Myogenic Engineered Tissue Constructs
13:43

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Published on: May 1, 2009

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Extruded alginate tubes with myogenic potential.

Cameron M Lenneman1, Emily M Rose1, Brooke A Strawska1

  • 1Physical Therapy Program. Department of Health Care Sciences. Eugene Applebaum College of Pharmacy and Health Sciences. Wayne State University. Detroit, MI, USA.

Biorxiv : the Preprint Server for Biology
|May 15, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed alginate tubes to deliver muscle stem cells (MuSCs) for muscle regeneration. These tubes support cell growth and can be implanted minimally invasively to potentially reverse muscle loss.

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Muscle Biology

Background:

  • Muscle loss due to trauma, disease, or aging lacks effective reversal methods.
  • Muscle satellite cells (MuSCs) are key to muscle regeneration.
  • Current methods for implanting MuSCs yield limited muscle regeneration.

Approach:

  • Developed a workflow to create cellularized alginate tubes resembling muscle tissue.
  • Prepared a 10% sodium alginate slurry with myogenic cells.
  • Extruded the slurry into calcium lactate to form tubes for implantation.

Key Points:

  • Alginate tubes possess suitable mechanical properties (1892 ± 527 mN peak stress, 14.17 ± 1.68 %/mm² Young's modulus) for minimally-invasive muscle embedding (MIME).
  • Implanted tubes supported cell seeding in mouse tibialis anterior muscle.
  • In vitro studies demonstrated tubes maintained MuSCs and facilitated myoblast outgrowth.

Conclusions:

  • Simple extrusion method generates alginate tubes with MuSCs/myoblasts.
  • Tubes are mechanically robust for minimally invasive implantation.
  • Cellularized tubes show myogenic potential, supporting cell growth in culture.