Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tight Junctions01:29

Tight Junctions

5.3K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.3K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Divergence in cellular markers observed in single-cell transcriptomics datasets between cultured primary trabecular meshwork cells and tissues.

Scientific data·2025
Same author

Influence of dexamethasone-induced matrices on the TM transcriptome.

Experimental eye research·2024
Same author

Characterization of extracellular matrix deposited by segmental trabecular meshwork cells.

Experimental eye research·2023
Same author

Characterization of extracellular matrix deposited by segmental trabecular meshwork cells.

bioRxiv : the preprint server for biology·2023
Same author

Anti-inflammatory and wound healing potential of medicinal maggot excretions/secretions at the ocular surface.

The ocular surface·2022
Same author

Metallic Engineered Nanomaterials and Ocular Toxicity: A Current Perspective.

Pharmaceutics·2022

Related Experiment Video

Updated: Jun 26, 2025

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.3K

To be or not to be - Decoding the Trabecular Meshwork Cell Identity.

Alice Tian1, Hasna Baidouri2, Sangbae Kim1,3,4

  • 1Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas 77030, USA.

Biorxiv : the Preprint Server for Biology
|May 15, 2024
PubMed
Summary

This study compares human trabecular meshwork (hTM) cells in culture versus tissue using single-cell RNA sequencing. Findings reveal differences in gene expression, impacting glaucoma research translation.

More Related Videos

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

6.6K
Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.0K

Related Experiment Videos

Last Updated: Jun 26, 2025

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.3K
Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

6.6K
Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.0K

Area of Science:

  • Ophthalmology
  • Genomics
  • Cell Biology

Background:

  • The human trabecular meshwork (hTM) is crucial for maintaining intraocular pressure (IOP).
  • In vitro hTM cell cultures are widely used but face challenges in translating findings to ex vivo or in vivo models due to cell heterogeneity.
  • A direct transcriptomic comparison of primary hTM cells in culture versus native tissue has not been previously reported.

Purpose of the Study:

  • To investigate the cellular identity and translatability of primary hTM cell cultures compared to hTM tissue.
  • To generate a comprehensive transcriptomic resource using single-cell RNA sequencing (scRNA-seq) of primary hTM cells.
  • To enable the study of global gene expression changes in hTM cells in vitro versus in situ.

Main Methods:

  • Collected 14 primary hTM in vitro samples (passages 1-4), including samples from 4 individuals with glaucoma.
  • Performed single-cell RNA sequencing (scRNA-seq) on primary hTM cell cultures.
  • Conducted extensive data preprocessing and quality control on the scRNA-seq dataset.

Main Results:

  • Generated a detailed transcriptomic profile of primary hTM cells in vitro.
  • Identified potential differences in cellular identity and gene expression between cultured hTM cells and hTM tissue.
  • Established a publicly accessible dataset for the research community.

Conclusions:

  • Primary hTM cell cultures exhibit significant transcriptomic differences compared to hTM tissue.
  • Understanding these differences is essential for accurate interpretation of in vitro glaucoma studies.
  • The generated dataset serves as a public resource to advance research on trabecular meshwork dysfunction and intraocular pressure regulation.