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Freezing mediastinal lymph node: first case of mediastinal cryobiopsy guided by EBUS in Brazil

João Pedro Steinhauser Motta1, Amir Szklo1, Bianca Peixoto Pinheiro Lucena1

  • 1. Instituto de Doenças do Tórax, Universidade Federal do Rio de Janeiro, Rio de Janeiro (RJ), Brasil.

Jornal Brasileiro De Pneumologia : Publicacao Oficial Da Sociedade Brasileira De Pneumologia E Tisilogia
|May 15, 2024
PubMed
Abstract

No abstract available in PubMed .

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...