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Variables Affecting Phosphorescence and Fluorescence01:26

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Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Absolute Quantum Yield Measurement of Powder Samples
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References for Small Fluorescence Quantum Yields.

Mahbobeh Morshedi1, Simon L Zimmermann1, David Klaverkamp1

  • 1Institut für Physikalische Chemie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany.

Journal of Fluorescence
|May 15, 2024
PubMed
Summary
This summary is machine-generated.

New reference compounds, including thymidine, dibenzoylmethane, and malachite green chloride, offer reliable determination of small fluorescence quantum yields across the UV/Vis spectrum. These photostable, commercially available materials provide accurate measurements for fluorescence spectroscopy applications.

Keywords:
DibenzoylmethaneFluorescence quantum yieldMalachite green chlorideStrickler-Berg analysisThymidineTime resolved spectroscopy

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Area of Science:

  • Analytical Chemistry
  • Photochemistry
  • Spectroscopy

Background:

  • Accurate determination of fluorescence quantum yields is crucial for various applications.
  • Existing references for low quantum yields are limited, especially across the UV/Vis spectrum.
  • Need for easily handled, photostable, and commercially available reference standards.

Purpose of the Study:

  • To introduce and validate three new compounds as reliable references for determining small fluorescence quantum yields.
  • To cover the blue, green, and red regions of the UV/Vis spectrum.
  • To assess the suitability of these compounds for routine fluorescence measurements.

Main Methods:

  • Relative fluorescence quantum yield determination.
  • Strickler-Berg analysis.
  • Time-resolved fluorescence spectroscopy.
  • Spectral analysis of absorption and emission spectra.

Main Results:

  • Thymidine (blue), dibenzoylmethane (green), and malachite green chloride (red) were identified as suitable references.
  • Fluorescence quantum yields were in the range of 10^-5 to 10^-4.
  • Compounds demonstrated photostability, ease of handling, and commercial availability.
  • Mirror-image symmetry between absorption and fluorescence spectra confirmed intrinsic emission.

Conclusions:

  • Thymidine, DBM, and MG are proposed as valuable new references for low fluorescence quantum yield determination.
  • The proposed compounds offer a reliable and accessible option for fluorescence spectroscopy.
  • The validated methods ensure accurate and consistent measurements across different spectral regions.