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Related Concept Videos

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  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Small Extracellular Vesicles Detection Using Dielectrophoresis-based Microfluidic Chip For Diagnosis Of Breast Cancer.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Small Extracellular Vesicles Detection Using Dielectrophoresis-based Microfluidic Chip For Diagnosis Of Breast Cancer.

Related Experiment Video

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
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Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

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Small extracellular vesicles detection using dielectrophoresis-based microfluidic chip for diagnosis of breast cancer.

Mei Lan1, Ze Ren1, Cheng Cheng1

  • 1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China.

Biosensors & Bioelectronics
|May 15, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

A new acDEP-Exo chip rapidly detects tumor biomarkers in small extracellular vesicles (sEVs) using minimal samples. This breakthrough offers highly accurate breast cancer detection through liquid biopsy.

Keywords:
BiomarkersBreast cancerDielectrophoresisLiquid biopsy

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biomarker Discovery

Background:

  • Small extracellular vesicles (sEVs) are valuable biomarkers for tumor diagnosis and monitoring.
  • Isolation and detection of sEVs from body fluids are challenging due to their small size and low density.

Purpose of the Study:

  • To develop a novel chip for rapid, sensitive, and tunable isolation and detection of sEVs.
  • To evaluate the chip's performance in detecting breast cancer biomarkers.

Main Methods:

  • A novel acDEP-Exo chip utilizing transparent micro-beads and a non-uniform electrical field was designed.
  • The chip requires a small sample volume (20-50 μL) and achieves biomarker detection within 13 minutes.
  • EpCAM and MUC1 markers on sEVs were analyzed in plasma samples from breast cancer patients and healthy volunteers.
Microfluidics
Small extracellular vesicles

Main Results:

  • The acDEP-Exo chip demonstrated a limit of detection of 161 particles/μL.
  • Combined evaluation of sEV biomarkers (EpCAM and MUC1) showed high sensitivity, specificity, and accuracy in breast cancer detection.
  • The method enables real-time analysis, crucial for timely clinical decisions.

Conclusions:

  • The acDEP-Exo chip provides an efficient and sensitive approach for sEV isolation and detection.
  • This technology holds significant potential for advancing liquid biopsy techniques for early cancer diagnosis and monitoring.
  • The study highlights the utility of sEV-based biomarkers for non-invasive cancer detection.