Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

70.6K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.6K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

62.1K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Curcumin Treatment is Associated with Increased Expression of the N-Methyl-D-Aspartate Receptor (NMDAR) Subunit, NR2A, in a Rat PC12 Cell Line Model of Alzheimer's Disease Treated with the Acetyl Amyloid-β Peptide, Aβ(25-35).

Medical science monitor : international medical journal of experimental and clinical research·2018
Same author

[Effects of Geometrical Dimensions and Material Properties on the Rotation Characteristics of Head].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi·2018
Same author

Molecular Mechanism of Loading Sulfur Hexafluoride in γ-Cyclodextrin Metal-Organic Framework.

The journal of physical chemistry. B·2018
Same author

Infrastructure Shapes Differences in the Carbon Intensities of Chinese Cities.

Environmental science & technology·2018
Same author

Post-transcription mediated Snail stabilization is involved in radiation exposure induced invasion and migration of hepatocarcinoma cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2018
Same author

Volatile organic compounds in stormwater from a community of Beijing, China.

Environmental pollution (Barking, Essex : 1987)·2018

Related Experiment Video

Updated: Jun 26, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.0K

A novel endolysin from an Enterococcus faecalis phage and application.

Yingying Xiang1, Suping Wang2, Hao Huang1

  • 1-Department of Stomatology, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650031, China.

Microbial Pathogenesis
|May 16, 2024
PubMed
Summary
This summary is machine-generated.

Phage endolysin pEF51 shows strong antibacterial activity against drug-resistant Enterococcus faecalis. This protein effectively removes biofilms and protects against infection in rat models, offering a potential alternative to antibiotics.

Keywords:
BiofilmEndolysinEnterococcus faecalisPhagePopulation

More Related Videos

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
11:33

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

Published on: August 17, 2017

14.7K
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.2K

Related Experiment Videos

Last Updated: Jun 26, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.0K
Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
11:33

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

Published on: August 17, 2017

14.7K
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Drug Discovery

Background:

  • Enterococcus faecalis is a primary cause of persistent infections after root canal therapy.
  • Antibiotic resistance in E. faecalis necessitates novel therapeutic strategies.
  • Phage endolysins are emerging as potential antibacterial agents.

Purpose of the Study:

  • To clone, express, and characterize the phage endolysin pEF51 for its antibacterial properties.
  • To evaluate the efficacy of pEF51 against E. faecalis planktonic cells and biofilms.
  • To assess the in vivo therapeutic potential of pEF51 in a rat infection model.

Main Methods:

  • Gene cloning and recombinant protein expression of phage endolysin pEF51.
  • Determination of antibacterial activity, lysis profile, and biofilm removal efficacy.
  • In vivo efficacy study using an infected Sprague-Dawley rat model and 16S rDNA analysis of gut microbiota.

Main Results:

  • Recombinant endolysin pEF51 exhibited significant antibacterial activity and a broad bactericidal spectrum.
  • pEF51 demonstrated high efficacy in eliminating E. faecalis biofilms.
  • In vivo treatment with pEF51 significantly reduced abscess formation in infected rats with minimal impact on gut microbial diversity.

Conclusions:

  • Phage endolysin pEF51 is a potent antibacterial agent against Enterococcus faecalis.
  • pEF51 shows promise as a therapeutic alternative to conventional antibiotics for E. faecalis infections.
  • Further research into pEF51's safety and efficacy is warranted for clinical applications.