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A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein-protein

Clinton G L Veale1, Abir Chakraborty2, Richwell Mhlanga2

  • 1Department of Chemistry, University of Cape Town, Rondebosch, Cape Town 7701, South Africa. clinton.veale@uct.ac.za.

Chemical Communications (Cambridge, England)
|May 16, 2024
PubMed

Native mass spectrometric analysis of TPR2A and GrpE with unpurified peptides derived from limited proteolysis of their respective PPI partners (HSP90 C-terminus and DnaK) facilitated efficient, qualitative identification of interfacial epitopes involved in transient PPI formation. Application of this approach can assist in elucidating interfaces of currently uncharacterised transient PPIs.

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