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Related Experiment Video

Updated: Jun 28, 2026

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs).

Lucie Descamps^1,2, Emmanuelle Laurenceau³, Sophie Cavassila⁴

¹Université Claude Bernard Lyon 1, CNRS, INSA Lyon, Ecole Centrale de Lyon, CPE Lyon, INL, Villeurbanne, France.

Methods in Molecular Biology (Clifton, N.J.)

|May 16, 2024

Summary

Isolating rare circulating tumor cells (CTCs) from blood is challenging. This study presents a microfluidic chip workflow for pure, live CTC recovery, aiding cancer monitoring and personalized treatments.

Keywords:

CTC recovery Circulating Tumor Cells (CTC)Composite polymer Immunomagnetic sorting Microfabrication Microfluidic chip Sample purification

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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

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Last Updated: Jun 28, 2026

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

Area of Science:

Biomedical Engineering
Oncology
Cellular Biology

Background:

Circulating tumor cells (CTCs) offer potential for cancer monitoring and personalized medicine.
Current methods face challenges in recovering rare CTCs with high purity and viability.
Microfluidic technology shows promise for addressing these limitations.

Purpose of the Study:

To develop and describe a novel microfluidic chip-based workflow for the isolation of pure, viable circulating tumor cells (CTCs).
To enable efficient CTC recovery for improved cancer diagnostics and treatment strategies.

Main Methods:

A two-step workflow combining size-based pre-enrichment with immunomagnetic purification using a microfluidic chip.
Detailed protocol for immunomagnetic microchip fabrication.
Sample preparation and microchip injection procedures for CTC sorting.

Main Results:

The described workflow facilitates the isolation of CTCs from whole blood.
The method aims to achieve high purity and viability of recovered CTCs.
The protocol is designed for compatibility with clinical timelines.

Conclusions:

The proposed microfluidic chip workflow represents a significant advancement in CTC isolation techniques.
This technology has the potential to enhance cancer monitoring and the development of personalized therapies.
Further validation is expected to confirm the clinical utility of this approach.