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Establishment of T-cell clones recognizing difference in H-2K antigen and inducing graft-versus-host disease
From the spleen cells of C57BL/6 (B6) mice, T-cell clones were established which responded to mutant H-2K antigen of B6. B-H-2bml (Hz1) mice. One clone and its subclones were maintained in culture medium containing lower concentration (2.5%) of IL-2 (culture supernatant of rat spleen cells stimulated with concanavalin A). They were shown to have a capability to induce graft-versus-host reaction in (B6 X Hz1)F1 recipients as well as to exert cytotoxicity against concanavalin-A-activated spleen cells of Hz1 mice and also their kidney fibroblasts. They lacked cytotoxicity against syngeneic and also third-party Con-A-stimulated spleen cells. These reactive clones were Thy-1.2 and Lyt-2 positive and Lyt-1 negative. On the other hand, clones which were maintained in culture medium containing higher concentration (20%) of IL-2 were devoid of activities in vivo as well as in vitro. These clones were Thy-1.2 positive but Lyt-1.2 and Lyt-2.2 negative.
From the spleen cells of C57BL/6 (B6) mice, T-cell clones were established which responded to mutant H-2K antigen of B6. B-H-2bml (Hz1) mice. One clone and its subclones were maintained in culture medium containing lower concentration (2.5%) of IL-2 (culture supernatant of rat spleen cells stimulated with concanavalin A). They were shown to have a capability to induce graft-versus-host reaction in (B6 X Hz1)F1 recipients as well as to exert cytotoxicity against concanavalin-A-activated spleen cells of Hz1 mice and also their kidney fibroblasts. They lacked cytotoxicity against syngeneic and also third-party Con-A-stimulated spleen cells. These reactive clones were Thy-1.2 and Lyt-2 positive and Lyt-1 negative. On the other hand, clones which were maintained in culture medium containing higher concentration (20%) of IL-2 were devoid of activities in vivo as well as in vitro. These clones were Thy-1.2 positive but Lyt-1.2 and Lyt-2.2 negative.