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Related Experiment Videos

Hexokinase in human chorionic villi.

M Magnani, G Novelli, V Stocchi

    Early Human Development
    |July 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Hexokinase (EC 2.7.1.1) isozymes were analyzed in early human placenta. The study identified multiple hexokinase types, explaining high placental glucose metabolism during pregnancy.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Human Physiology

    Background:

    • Hexokinase (EC 2.7.1.1) is crucial for glucose metabolism.
    • Understanding its role in early pregnancy is vital for placental development.

    Purpose of the Study:

    • To determine the level, intracellular distribution, and isozymic pattern of hexokinase in first-trimester human chorionic villi.
    • To elucidate the contribution of different hexokinase isozymes to placental glucose utilization.

    Main Methods:

    • Trophoblast biopsy for human chorionic villi collection.
    • Enzyme assays to determine hexokinase activity, distribution, and kinetic properties.
    • Chromatographic and kinetic analyses to identify hexokinase isozymes.

    Main Results:

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    • Approximately 50% of total hexokinase activity was particle-bound, with 96% in overt form.
    • Soluble and particulate hexokinase exhibited similar glucose affinity but differed in MgATP2- affinity and glucose 1,6-diphosphate inhibition sensitivity.
    • Soluble hexokinase comprised isozymes I, II, and traces of III; bound enzyme was predominantly hexokinase type I.

    Conclusions:

    • At least three hexokinase isozymes are expressed in early human placental development.
    • The identified hexokinase isozymic pattern contributes to the high glucose utilization rate observed in the placenta.
    • Findings provide insights into the biochemical adaptations supporting early embryonic and fetal growth.