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Related Experiment Video

Updated: Jun 26, 2025

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

Prohibitin modulates periodontium differentiation in mice development.

Yam Prasad Aryal^1,2, Song-Yi Han³, Bandana Rana¹

¹Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Republic of Korea.

Frontiers in Cell and Developmental Biology

|May 17, 2024

Summary

Prohibitin (PHB) is crucial for alveolar bone formation during periodontium development. Knocking down PHB in mice reduced bone mass and altered key signaling pathways, highlighting its regulatory role.

Keywords:

alveolar bone process differentiation functional evaluation microinjecfion periodontium

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Published on: February 13, 2021

Area of Science:

Developmental Biology
Periodontology
Molecular Biology

Background:

Prohibitin (PHB) is a scaffold protein involved in cell survival, metabolism, inflammation, and bone formation.
The specific role of PHB in periodontium development and alveolar bone formation is not well understood.

Purpose of the Study:

To investigate the expression pattern of PHB in the developing periodontium.
To elucidate the function of PHB in alveolar bone formation and periodontal ligament differentiation.

Main Methods:

Immunolocalization of PHB in postnatal mouse periodontium.
Phb morpholino micro-injection in mouse mandibles to assess PHB function.
Immunohistochemistry, micro-CT evaluation, and in vitro dental mesenchyme culture to analyze bone formation and signaling pathways.

Main Results:

PHB is localized in the periodontium during postnatal development.
Phb knockdown resulted in decreased bone mass, increased periodontal ligament space, and altered expression of bone-related markers (RUNX2, OCN).
PHB downregulation affected Bmps, Runx2, and Wnt signaling pathways in developing dental mesenchyme.

Conclusions:

PHB plays a significant role in modulating alveolar bone formation during periodontium development.
PHB influences the differentiation of periodontal ligament and regulates key bone-forming signaling pathways.