Related Experiment Video

Updated: May 12, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

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Changes in the intestinal microbiota of multiple myeloma patients living in high‑altitude and cold regions analyzed

Xiaofei Liang1, Xuyang Guo1, Huixin Jin1

  • 1Department of Laboratory Medicine, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161000, P.R. China.

Experimental and Therapeutic Medicine
|May 17, 2024
PubMed

Insights

Multiple myeloma (MM) patients show distinct gut microbiota profiles, with specific bacteria enriched in the gut. This gut dysbiosis may offer novel therapeutic targets for MM treatment.

Area of Science:

  • Microbiology
  • Oncology
  • Genetics

Background:

  • Multiple myeloma (MM) is a plasma cell malignancy where malignant cells can persist in the gut.
  • Gut microbiota dysfunction can release stimuli promoting genetic mutations and clonal evolution.
  • Understanding the gut microbiota's role in MM is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To analyze the intestinal microbiota composition in multiple myeloma patients from high-altitude and cold regions of China.
  • To identify specific bacterial species and pathways associated with MM.
  • To explore the potential of the gut microbiota as a therapeutic target for MM.

Main Methods:

  • 16S rRNA sequencing of fecal samples from MM patients and controls.
  • Analysis of alpha and beta diversity of the gut microbiota.
  • Random Forest and ROC analysis for disease diagnosis model construction.
  • Correlation analysis with complement levels and KEGG pathway analysis.

Main Results:

  • No significant difference in alpha diversity, but significant differences in beta diversity between MM and control groups.
  • Fifteen differentially abundant bacterial genera identified, with *Bacteroides*, *Streptococcus*, *Lactobacillus*, and *Alistipes* enriched in MM patients.
  • A diagnostic model for MM was developed with high accuracy.
  • Gut microbiota composition correlated with complement levels and predicted affected signaling and metabolic pathways in MM.

Conclusions:

  • The gut microbiota composition is significantly altered in multiple myeloma patients.
  • Specific bacterial taxa and metabolic pathways in the gut are associated with MM progression.
  • The gut microbiota represents a potential novel therapeutic target for multiple myeloma.
Keywords:
16s rRNAKyoto Encyclopedia of Genes and Genomescomplementgutintestinal microbiotamultiple myeloma

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