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Updated: Jun 26, 2025

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA
Nicholas F Chong1, Alexander Idnurm2, Bridgit C Nugent1
1School of BioSciences, The University of Melbourne, Parkville, VIC, Australia.
Abstract:
Transformation of foreign DNA into Cryptococcus species is a powerful tool for exploring gene functions in these human pathogens. Agrobacterium tumefaciens-mediated transformation (AtMT) has been used for the stable introduction of exogenous DNA into Cryptococcus for over two decades, being particularly impactful for insertional mutagenesis screens to discover new genes involved in fungal biology. A detailed protocol to conduct this transformation method is provided in the chapter. Scope for modifications and the benefits and disadvantages of using AtMT in Cryptococcus species are also presented.

