Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracellular <i>C. neoformans</i> infection stimulates increased glycolytic activity in fetal liver-derived alveolar-like macrophages.

bioRxiv : the preprint server for biology·2025
Same author

Isolation and characterization of a Chlamydia muridarum tc0237 mutant from a genetic screen that is attenuated in epithelial cells.

PloS one·2025
Same author

Editorial: Exploring the molecular mechanisms that regulate macrophage polarization.

Frontiers in immunology·2025
Same author

Noninvasive Monitoring of Humoral Immune Responses in Men With Acute Chlamydia trachomatis Urethral Infection Using First-Catch Urine.

The Journal of infectious diseases·2025
Same author

The Impact of Testosterone Therapy on the Vaginal Microbiota of Transgender Men and Nonbinary People: A Prospective Study.

The Journal of infectious diseases·2025
Same author

Tail-specific protease is an essential <i>Chlamydia</i> virulence factor that mediates the differentiation of elementary bodies into reticulate bodies.

Infection and immunity·2024

Related Experiment Video

Updated: Jun 26, 2025

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
08:24

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Published on: February 27, 2018

13.9K

Quantification of C. neoformans Capsule Diameter.

Tiffany E Guess1,2, Hoyin Lai3, David E Nelson4

  • 1Middle Tennessee State University, Murfreesboro, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2024
PubMed
Summary

The polysaccharide capsule of Cryptococcus neoformans is key to its virulence. This study details methods for inducing capsule growth and measuring its size using India ink or fluorescent staining, aiding research into fungal pathogenesis.

Keywords:
CapsuleCryptococcus neoformansFluorescent stainImage analysisIndia inkVirulenceYeast

More Related Videos

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
08:08

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

7.3K
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
10:02

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

Published on: October 21, 2014

11.8K

Related Experiment Videos

Last Updated: Jun 26, 2025

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
08:24

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Published on: February 27, 2018

13.9K
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
08:08

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

7.3K
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
10:02

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

Published on: October 21, 2014

11.8K

Area of Science:

  • Mycology
  • Pathogenic Microbiology
  • Cell Biology

Background:

  • The polysaccharide capsule of Cryptococcus neoformans is a critical virulence factor.
  • Capsule size is variable, correlates with virulence, and grows under stress.
  • Understanding capsule dynamics is vital for C. neoformans research.

Purpose of the Study:

  • To describe standard methods for inducing Cryptococcus neoformans capsule growth.
  • To detail established staining techniques for capsule visualization.
  • To present protocols for both manual and automated capsule diameter measurement.

Main Methods:

  • Capsule induction protocols for C. neoformans.
  • Staining methods including India ink (negative stain) and fluorescent co-staining.
  • Microscopy techniques: light and confocal microscopy.
  • Manual and computational image analysis for capsule diameter measurement.

Main Results:

  • Standardized methods for capsule induction and visualization are presented.
  • Two distinct staining and microscopy approaches (India ink/light microscopy, fluorescent dyes/confocal microscopy) are detailed.
  • Protocols for manual and automated capsule size measurement are provided.

Conclusions:

  • Accurate measurement of C. neoformans capsule size is crucial for virulence studies.
  • The described methods facilitate phenotypic testing and strain comparison.
  • Automated image analysis offers an efficient approach for quantifying capsule diameter.