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Overview of Secretory Vesicles

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

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Related Experiment Video

Updated: Jun 26, 2025

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Analysis of Cryptococcus Extracellular Vesicles.

Flavia C G Reis^1,2, Rafael F Castelli¹, Diogo Kuczera¹

¹Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.

Methods in Molecular Biology (Clifton, N.J.)

|May 17, 2024

Summary

This summary is machine-generated.

This study presents a rapid method for isolating fungal extracellular vesicles (EVs) from Cryptococcus. This breakthrough simplifies the study of these vital microbial components in fungal pathogens.

Keywords:

Cryptococcus Electron microscopy Extracellular vesicles Nanoparticle tracking analysis Solid media

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Last Updated: Jun 26, 2025

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Area of Science:

Mycology
Microbiology
Cell Biology

Background:

Extracellular vesicles (EVs) are crucial in fungal pathogenesis, influencing virulence and immune responses.
Cryptococcus EVs play significant roles in fungal virulence, drug resistance, and host immunity.
Traditional methods for isolating fungal EVs are often inefficient and time-consuming.

Purpose of the Study:

To introduce a streamlined and efficient protocol for isolating EVs from Cryptococcus species.
To facilitate research into the biological functions of Cryptococcus EVs.

Main Methods:

Detailed description of a novel, rapid protocol for EV isolation from Cryptococcus.
Methodology focuses on speed and efficiency compared to existing techniques.

Main Results:

Successful isolation of Cryptococcus EVs using the developed protocol.
The protocol significantly reduces the time and labor required for EV preparation.

Conclusions:

The presented protocol offers a practical solution for researchers studying Cryptococcus EVs.
This advancement will accelerate investigations into fungal pathogenesis and immune interactions.