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Probiotic Pediococcus acidilactici Strains Exert Anti-inflammatory Effects by Regulating Intracellular Signaling
Im-Kyung Woo1, Jun-Hyun Hyun1, Hye Ji Jang1
1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, Republic of Korea.
Abstract:
This study investigated the anti-inflammatory effects of Pediococcus acidilactici strains isolated from fermented vegetables on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. In addition, the probiotic characteristics and safety were evaluated. Our results show that Ped. acidilactici strains possess high survivability in simulated gastrointestinal environments and strong attachment to HT-29 cells. All Ped. acidilactici strains exhibited γ-hemolysis and resistance to gentamicin, kanamycin, and streptomycin, a characteristic commonly observed in lactic acid bacteria. Treatment with Ped. acidilactici inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2, leading to a subsequent reduction in nitric oxide and prostaglandin E2 production. Furthermore, the strains downregulated interleukin (IL)-1β and IL-6 mRNA levels, ultimately suppressing their production. We demonstrated that Ped. acidilactici strains could modulate the activation of nuclear factor-κB, mitogen-activated protein kinase, and activator protein-1, which are known to regulate inflammatory responses. Consequently, the anti-inflammatory properties of Ped. acidilactici strains in this study support their potential application as therapeutic agents for inflammatory diseases, providing molecular insights into next-generation functional probiotic products.
Insights
Pediococcus acidilactici from fermented vegetables show probiotic potential. These beneficial bacteria reduce inflammation by inhibiting key inflammatory molecules and pathways, supporting their use in functional foods and therapeutics.
Area of Science:
- Microbiology
- Immunology
- Gastroenterology
Background:
- Inflammatory diseases pose a significant health burden.
- Probiotics offer a potential avenue for managing inflammation.
- Pediococcus acidilactici, a lactic acid bacterium, is found in fermented foods.
Purpose of the Study:
- To evaluate the anti-inflammatory effects of Pediococcus acidilactici strains.
- To assess the probiotic characteristics and safety of these strains.
- To elucidate the molecular mechanisms underlying their anti-inflammatory action.
Main Methods:
- Isolated Pediococcus acidilactici strains from fermented vegetables.
- Assessed survivability in simulated gastrointestinal conditions and adhesion to HT-29 cells.
- Measured inhibition of inflammatory mediators (nitric oxide, prostaglandin E2, IL-1β, IL-6) in LPS-stimulated RAW 264.7 macrophages.
- Analyzed modulation of key inflammatory signaling pathways (NF-κB, MAPK, AP-1).
Main Results:
- Pediococcus acidilactici strains demonstrated excellent gastrointestinal survivability and cell adhesion.
- Strains showed characteristic lactic acid bacteria safety markers (γ-hemolysis, antibiotic resistance).
- Significant reduction in nitric oxide, prostaglandin E2, IL-1β, and IL-6 production was observed.
- Modulation of NF-κB, MAPK, and AP-1 signaling pathways confirmed.
Conclusions:
- Pediococcus acidilactici strains possess significant anti-inflammatory properties.
- These strains exhibit robust probiotic characteristics and safety profiles.
- The findings support the potential of Pediococcus acidilactici as therapeutic agents for inflammatory conditions.
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