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CaptureSelect FcXP affinity medium exhibits strong aggregate separation capability.

Wanyuan Dong1, Dan Zhang1, Yifeng Li1

  • 1Downstream Process Development (DSPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.

Protein Expression and Purification
|May 17, 2024
PubMed
Summary
This summary is machine-generated.

CaptureSelect FcXP affinity medium effectively removes aggregates during bispecific antibody purification, offering a potential alternative to Protein A for high-aggregate feed streams. This improves antibody product purity.

Keywords:
AggregateBispecific antibody (bsAb)CaptureSelect FcXPConductivity gradientProtein ApH gradient

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Area of Science:

  • Biotechnology
  • Biopharmaceutical Manufacturing
  • Protein Purification

Background:

  • Protein A chromatography is a standard for antibody purification but struggles with aggregate removal.
  • Aggregate removal is critical for antibody efficacy and safety.
  • Existing methods for aggregate separation can be complex or inefficient.

Purpose of the Study:

  • To evaluate the aggregate separation capability of CaptureSelect FcXP affinity medium.
  • To compare FcXP's performance against Protein A for bispecific antibody purification.
  • To assess FcXP's effectiveness in reducing aggregate content in feed streams.

Main Methods:

  • Utilized CaptureSelect FcXP affinity medium for bispecific antibody purification.
  • Employed pH and conductivity gradient elution strategies.
  • Quantified aggregate content in feed and eluate samples using analytical techniques.

Main Results:

  • FcXP demonstrated strong aggregate separation capability in two bispecific antibody cases.
  • Aggregate content was significantly reduced from over 16% and 22% in the feed to below 1% and 5% in the eluate.
  • Effective aggregate removal was achieved under gradient elution conditions.

Conclusions:

  • CaptureSelect FcXP affinity medium shows significant potential for aggregate removal during bispecific antibody purification.
  • FcXP may be a superior alternative to Protein A for product capture when dealing with high aggregate loads.
  • Further studies are warranted to fully establish FcXP's advantages in aggregate removal.