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Simultaneous multiple target detection platform based on vertical flow immunoassay.

Taek Yong1, Dami Kim2, Sanghyo Kim3

  • 1Department of Bionanotechnology, Gachon University, Seongnam 13120, Republic of Korea.

Journal of Immunological Methods
|May 17, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel paper-based vertical flow assay (VFA) device simplifying multi-target detection. The innovative design enables a one-step analysis process for simultaneous detection of biomarkers like IgG and CRP.

Keywords:
Antigen-antibody bindingGold nanoparticlesMultiple detectionOne-step detectionVertical flow assay

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Area of Science:

  • Biomarker detection
  • Assay development
  • Paper-based diagnostics

Background:

  • Traditional vertical flow assays (VFAs) involve complex, multi-step reagent application.
  • Existing methods can be time-consuming and labor-intensive for analyzing multiple targets.

Purpose of the Study:

  • To develop an innovative paper-based VFA device for simplified, one-step, multi-target detection.
  • To evaluate the device's performance using common biomarkers, Immunoglobulin G (IgG) and C-reactive protein (CRP).

Main Methods:

  • Fabrication of a paper-based VFA device with a cartridge for uniform array formation.
  • Optimization of sample flow rates, analysis time, and limit of detection (LOD).
  • Validation using simultaneous detection of human IgG and CRP antigens-antibodies, assessing cross-reactivity.

Main Results:

  • The developed paper-based VFA device enables a simplified one-step analysis process.
  • Simultaneous detection of multiple targets (IgG and CRP) was achieved with naked-eye LODs of 0.15 μg/mL for IgG and 0.19 μg/mL for CRP.
  • No cross-reactivity was observed between IgG and CRP detection, confirming device specificity.

Conclusions:

  • The novel paper-based VFA device offers a cost-effective and efficient solution for multi-target biomarker detection.
  • The one-step analysis and simultaneous detection capabilities represent a significant advancement over traditional VFA methods.
  • This technology holds potential for rapid and accessible diagnostic applications.